Cloned (Comment) | Organism |
---|---|
gene cloning, DNA and amino acid sequence determination and analysis, the At4g35160 gene exhibits the highest sequence identity (31%) to the rice ASMT gene, followed by the At1g76790 gene with 29% sequence identity, phylogenetic analysis. The recombinant proteins are recombinantly expressed from the two Arabidopsis genes. The At4g35160 recombinant protein exhibits ASMT enzyme activity, but the At1g76790 recombinant protein does not. Recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | ectopic overexpression of the enzyme in rice results in increased ASMT enzyme activity and melatonin production | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by caffeic acid | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.456 | - |
N-acetylserotonin | pH 7.8, 37°C | Arabidopsis thaliana | |
1.036 | - |
serotonin | pH 8.8, 37°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Arabidopsis thaliana | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | the AtASMT protein catalyzes the conversion of N-acetylserotonin to melatonin and serotonin to 5-methoxytryptamine, respectively. The enzyme exhibits no caffeic acid O-methyltransferase activity, suggesting that its function is highly specific to melatonin synthesis | ? | - |
? | |
additional information | Arabidopsis thaliana Col-0 | the AtASMT protein catalyzes the conversion of N-acetylserotonin to melatonin and serotonin to 5-methoxytryptamine, respectively. The enzyme exhibits no caffeic acid O-methyltransferase activity, suggesting that its function is highly specific to melatonin synthesis | ? | - |
? | |
S-adenosyl-L-methionine + N-acetylserotonin | Arabidopsis thaliana | - |
S-adenosyl-L-homocysteine + melatonin | - |
? | |
S-adenosyl-L-methionine + N-acetylserotonin | Arabidopsis thaliana Col-0 | - |
S-adenosyl-L-homocysteine + melatonin | - |
? | |
S-adenosyl-L-methionine + serotonin | Arabidopsis thaliana | enzyme AtASMT also O-methylates serotonin to produce 5-methoxytryptamine | S-adenosyl-L-homocysteine + 5-methoxytryptamine | - |
? | |
S-adenosyl-L-methionine + serotonin | Arabidopsis thaliana Col-0 | enzyme AtASMT also O-methylates serotonin to produce 5-methoxytryptamine | S-adenosyl-L-homocysteine + 5-methoxytryptamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9T003 | gene At4g35160 or AtASMT; gene AtASMT | - |
Arabidopsis thaliana Col-0 | Q9T003 | gene At4g35160 or AtASMT; gene AtASMT | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000007 | - |
purified recombinant enzyme, pH 7.8, 37°C | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the AtASMT protein catalyzes the conversion of N-acetylserotonin to melatonin and serotonin to 5-methoxytryptamine, respectively. The enzyme exhibits no caffeic acid O-methyltransferase activity, suggesting that its function is highly specific to melatonin synthesis | Arabidopsis thaliana | ? | - |
? | |
additional information | caffeic acid is no substrate | Arabidopsis thaliana | ? | - |
? | |
additional information | the AtASMT protein catalyzes the conversion of N-acetylserotonin to melatonin and serotonin to 5-methoxytryptamine, respectively. The enzyme exhibits no caffeic acid O-methyltransferase activity, suggesting that its function is highly specific to melatonin synthesis | Arabidopsis thaliana Col-0 | ? | - |
? | |
additional information | caffeic acid is no substrate | Arabidopsis thaliana Col-0 | ? | - |
? | |
S-adenosyl-L-methionine + N-acetylserotonin | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + melatonin | - |
? | |
S-adenosyl-L-methionine + N-acetylserotonin | - |
Arabidopsis thaliana Col-0 | S-adenosyl-L-homocysteine + melatonin | - |
? | |
S-adenosyl-L-methionine + serotonin | enzyme AtASMT also O-methylates serotonin to produce 5-methoxytryptamine | Arabidopsis thaliana | S-adenosyl-L-homocysteine + 5-methoxytryptamine | - |
? | |
S-adenosyl-L-methionine + serotonin | enzyme AtASMT also O-methylates serotonin to produce 5-methoxytryptamine | Arabidopsis thaliana Col-0 | S-adenosyl-L-homocysteine + 5-methoxytryptamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ASMT | - |
Arabidopsis thaliana |
N-acetylserotonin O-methyltransferase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
O-methylation of N-acetylserotonin | Arabidopsis thaliana |
8.8 | - |
O-methylation of serotonin | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | AtASMT transcripts are induced by cadmium treatment in Arabidopsis thaliana followed by increased melatonin synthesis | up |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the conversion of N-acetylserotonin to melatonin as the last step in melatonin biosynthesis. The enzyme exhibits no caffeic acid O-methyltransferase activity, suggesting that its function is highly specific to melatonin synthesis | Arabidopsis thaliana |