Cloned (Comment) | Organism |
---|---|
gene comt, recombinant expression in Escherichia coli strain BL21(DE3), the N-terminal His-tagged AtCOMT is more highly expressed in soluble form than the C-terminal His-tagged AtCOMT | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an Arabidopsis thaliana comt knockout mutant (SALK_002373) with T-DNA inserted at the third exon. The comt mutant shows no detectable COMT transcript and exhibits a 20fold less COMT activity relative over the wild type, the phenotypes such as growth and fertility are indistinguishable from the wild-type plants | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.233 | - |
N-acetylserotonin | pH 7.8, 30°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Arabidopsis thaliana | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. COMT is a multifunctional enzyme methylating a diverse array of substrates, including phenylpropanoids, flavonoids, and arylalkylamines | ? | - |
? | |
additional information | Arabidopsis thaliana Col-0 | caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. COMT is a multifunctional enzyme methylating a diverse array of substrates, including phenylpropanoids, flavonoids, and arylalkylamines | ? | - |
? | |
S-adenosyl-L-methionine + N-acetylserotonin | Arabidopsis thaliana | - |
S-adenosyl-L-homocysteine + melatonin | - |
? | |
S-adenosyl-L-methionine + N-acetylserotonin | Arabidopsis thaliana Col-0 | - |
S-adenosyl-L-homocysteine + melatonin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9T003 | gene At4g35160 or AtASMT; gene AtASMT | - |
Arabidopsis thaliana Col-0 | Q9T003 | gene At4g35160 or AtASMT; gene AtASMT | - |
Purification (Comment) | Organism |
---|---|
reombinant N-and C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatograpy | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. COMT is a multifunctional enzyme methylating a diverse array of substrates, including phenylpropanoids, flavonoids, and arylalkylamines | Arabidopsis thaliana | ? | - |
? | |
additional information | caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. COMT is a multifunctional enzyme methylating a diverse array of substrates, including phenylpropanoids, flavonoids, and arylalkylamines | Arabidopsis thaliana Col-0 | ? | - |
? | |
S-adenosyl-L-methionine + N-acetylserotonin | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + melatonin | - |
? | |
S-adenosyl-L-methionine + N-acetylserotonin | - |
Arabidopsis thaliana Col-0 | S-adenosyl-L-homocysteine + melatonin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ASMT | - |
Arabidopsis thaliana |
N-acetylserotonin methyltransferase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
both recombinant N- and C-terminal fusions of enzyme AtCOMT exhibit the highest enzyme activities at pH 37°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
both recombinant N- and C-terminal fusions of enzyme AtCOMT exhibit the highest enzyme activities at pH 7.8 | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.8 | both recombinant N- and C-terminal fusions of the enzyme, no activity at pH 5.4 | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | in vivo role played by the COMT for biosynthesizing melatonin | Arabidopsis thaliana |