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Literature summary for 2.1.1.4 extracted from

  • Axelrod, J.; Weissbach, H.
    Purification and properties of hydroxyindole-O-methyl transferase (1961), J. Biol. Chem., 236, 211-213.
    View publication on PubMed
Search for more literature for 2.1.1.4

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoate
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.046
-
 S-adenosyl-L-methionine
-
 Bos taurus
0.054
-
 N-acetylserotonin
-
 Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetylserotonin + S-adenosyl-L-methionine Bos taurus formation of the hormone melatonin S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
 ?
N-acetylserotonin + S-adenosyl-L-methionine Felis catus formation of the hormone melatonin S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
 ?
N-acetylserotonin + S-adenosyl-L-methionine Macaca mulatta formation of the hormone melatonin S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Felis catus
-
-
-
Macaca mulatta
-
 monkey
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain not Bos taurus
-
brain not Felis catus
-
brain not Macaca mulatta
-
pineal gland
-
 Bos taurus
-
pineal gland
-
 Felis catus
-
pineal gland
-
 Macaca mulatta
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 enzyme assay Bos taurus
additional information
-
 enzyme assay Felis catus
additional information
-
 enzyme assay Macaca mulatta

Storage Stability

Storage Stability Organism
frozen, stable for several weeks Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxy-N-acetyltryptamine + S-adenosyl-L-methionine 8% of the activity with N-acetylserotonin Bos taurus S-adenosyl-L-homocysteine + N-acetyl-4-methoxytryptamine
-
 ?
5-hydroxyindoleacetic acid + S-adenosyl-L-methionine 12% of the activity with N-acetylserotonin Bos taurus S-adenosyl-L-homocysteine + 5-methoxyindoleacetic acid
-
 ?
bufotenine + S-adenosyl-L-methionine 14% of the activity with N-acetylserotonin Bos taurus S-adenosyl-L-homocysteine + N,N-dimethyl-5-hydroxytryptamine
-
 ?
additional information absolute requirement for S-adenosylmethionine Bos taurus ?
-
 ?
additional information absolute requirement for S-adenosylmethionine Felis catus ?
-
 ?
additional information absolute requirement for S-adenosylmethionine Macaca mulatta ?
-
 ?
N-acetylserotonin + S-adenosyl-L-methionine formation of the hormone melatonin Bos taurus S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
 ?
N-acetylserotonin + S-adenosyl-L-methionine formation of the hormone melatonin Felis catus S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
 ?
N-acetylserotonin + S-adenosyl-L-methionine formation of the hormone melatonin Macaca mulatta S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
 ?
N-methylserotonin + S-adenosyl-L-methionine 9% of the activity with N-acetylserotonin Bos taurus S-adenosyl-L-homocysteine + N-methyl-5-methoxytryptamine
-
 ?
S-adenosyl-L-methionine + N-acetylserotonin
-
 Bos taurus S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
 ?
S-adenosyl-L-methionine + N-acetylserotonin
-
 Felis catus S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
 ?
S-adenosyl-L-methionine + N-acetylserotonin
-
 Macaca mulatta S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
 ?
serotonin + S-adenosyl-L-methionine 7% of the activity with N-acetylserotonin Bos taurus S-adenosyl-L-homocysteine + 5-methoxytryptamine
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.3
-
 Bos taurus