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Literature summary for 2.1.1.37 extracted from

  • Pradhan, M.; Esteve, P.O.; Chin, H.G.; Samaranayke, M.; Kim, G.D.; Pradhan, S.
    CXXC domain of human DNMT1 is essential for enzymatic activity (2008), Biochemistry, 47, 10000-10009.
    View publication on PubMed
Search for more literature for 2.1.1.37

Cloned(Commentary)

Cloned (Comment) Organism
DNMT1, overexpression of HA-tagged CXXC deletion DNMT1 mutant, expression of wild-type and deletion mutant enzymes in COS-7 cells and Escherichia coli, CXXC deleted DNMT1, mutant DNMT1DELTACXXC, is localized on replication foci Homo sapiens

Protein Variants

Protein Variants Comment Organism
C667G zinc-finger-domain point mutation in the CXXC motif abolishes unmethylated DNA binding activity Homo sapiens
C667G/C670G zinc-finger-domain point mutation in the CXXC motif abolishes unmethylated DNA binding activity Homo sapiens
C670G zinc-finger-domain point mutation in the CXXC motif abolishes unmethylated DNA binding activity Homo sapiens
additional information construction of zinc-finger-domain point mutant and deletion mutant DNMT1 clones, CXXC deleted DNMT1, i.e. mutant DNMT1DELTACXXC and DNMT1 lacking the first 580 amino acids, i.e. DNMT1DELTA580. The latter prefers binding to unmethylated DNA instead of hemimethylated or fully methylated DNA like the wild-type enzyme. Deletion of CXXC domain in DNMT1 does not affect replication foci occupancy during DNA replication. A permanent cell line with DNMT1DELTACXXC displays partial loss of genomic methylation on rDNA loci, despite the presence of endogenous wild-type enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + S-adenosyl-L-methionine Homo sapiens DNA cytosine methylation is one of the major epigenetic gene silencing marks in the human genome facilitated by DNA methyltransferases DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P26358
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + S-adenosyl-L-methionine DNA cytosine methylation is one of the major epigenetic gene silencing marks in the human genome facilitated by DNA methyltransferases Homo sapiens DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
 ?
DNA + S-adenosyl-L-methionine poly(dI-dC) substrate DNA. The enzyme contains the zinc-finger CXXC domain, a zinc-coordinating domain that selectively binds unmethylated DNA and is crucial for enzymatic activity Homo sapiens DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
 ?

Subunits

Subunits Comment Organism
More the conserved CXXC domain encompassing the amino terminus region of DNMT1 cooperates with the catalytic domain for DNA methyltransferase activity, and binds to unmethylated CpG Homo sapiens

Synonyms

Synonyms Comment Organism
DNA cytosine-5 methyltransferase 1
-
 Homo sapiens
Dnmt1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Homo sapiens