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Literature summary for 2.1.1.37 extracted from

  • Gowher, H.; Liebert, K.; Hermann, A.; Xu, G.; Jeltsch, A.
    Mechanism of stimulation of catalytic activity of Dnmt3A and Dnmt3B DNA-(cytosine-C5)-methyltransferases by Dnmt3L (2005), J. Biol. Chem., 280, 13341-13348.
    View publication on PubMed
Search for more literature for 2.1.1.37

Activating Compound

Activating Compound Comment Organism Structure
DNA(cytosine-S)-methyltransferase 3-like protein i.e. Dnmt3L, UniProt Acc Q09CWR8. Dnmt3L stimulates activity of Dnmt3B by directly binding to the catalytic domain via its own C-terminal domain. The catalytic activity is stimulated about 15fold. Dnmt3L directly binds to DNA but not to S-adenosyl-L-methionine. Dnmt3L acts as a substrate exchange factor that accelerates DNA and AdoMet binding Mus musculus
DNA(cytosine-S)-methyltransferase 3-like protein i.e. Dnmt3L, UniProt Acc Q09CWR8. Dnmt3L stimulates activity of the Dnmt3A by directly binding to the catalytic domain via its own C-terminal domain. The catalytic activity is stimulated about 15fold. Dnmt3L directly binds to DNA but not to S-adenosyl-L-methionine. Complex formation between Dnmt3A and Dnmt3L accelerates DNA binding by Dnmt3A 20fold and lowers its Km for DNA. Interaction of Dnmt3L with Dnmt3A increases the binding of AdoMet to Dnmt3A, and lowers the Km-value of Dnmt3A for AdoMet. Interaction of Dnmt3A and Dnmt3L is transient, and after DNA binding to Dnmt3A, Dnmt3L dissociates from the complex. Following dissociation of Dnmt3L, Dnmt3A adopts a closed conformation leading to slow rates of DNA release. Therefore, Dnmt3L acts as a substrate exchange factor that accelerates DNA and AdoMet binding Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00025
-
 DNA enzyme Dnmt3A, in presence of Dnmt3L Mus musculus
0.00076
-
 DNA enzyme Dnmt3A, in absence of Dnmt3L Mus musculus
0.00096
-
 S-adenosyl-L-methionine enzyme Dnmt3A, in presence of Dnmt3L Mus musculus
0.00255
-
 S-adenosyl-L-methionine enzyme Dnmt3A, in absence of Dnmt3L Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus O88508 recombinant, enzyme Dnmt3A
-
Mus musculus O88509 recombinant, enzyme Dnmt3B
-

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + DNA
-
 Mus musculus S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 S-adenosyl-L-methionine enzyme Dnmt3A, in absence of Dnmt3L Mus musculus
0.01
-
 DNA enzyme Dnmt3A, in absence of Dnmt3L Mus musculus
0.013
-
 S-adenosyl-L-methionine enzyme Dnmt3A, in absence of Dnmt3L Mus musculus
0.013
-
 DNA enzyme Dnmt3A, in presence of Dnmt3L Mus musculus