Cloned (Comment) | Organism |
---|---|
expression in Sf9 cells | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000273 | - |
unmethylated CpG | pH 7.0 | Nicotiana tabacum | |
0.000037 | - |
hemimethylated CpG | pH 7.0 | Nicotiana tabacum | |
0.0000459 | - |
CpNpN | pH 7.0 | Nicotiana tabacum | |
0.0000783 | - |
CpNpG | pH 7.0 | Nicotiana tabacum | |
0.00258 | - |
poly(dI-dC) | pH 7.0 | Nicotiana tabacum | |
0.00636 | - |
poly(dG-dC) | pH 7.0 | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | exclusively in | Nicotiana tabacum | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA | Nicotiana tabacum | NtDRM1 is a de novo cytosine methyltransferase which actively excludes CpG substrate | S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
cv. Xanthi | - |
Purification (Comment) | Organism |
---|---|
- |
Nicotiana tabacum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Nicotiana tabacum | - |
leaf | - |
Nicotiana tabacum | - |
root | - |
Nicotiana tabacum | - |
stem | - |
Nicotiana tabacum | - |
TBY-2 cell | - |
Nicotiana tabacum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + CpG | - |
Nicotiana tabacum | S-adenosyl-L-homocysteine + CpG containing 5-methylcytosine | - |
? | |
S-adenosyl-L-methionine + CpNpG | - |
Nicotiana tabacum | ? | - |
? | |
S-adenosyl-L-methionine + CpNpN | - |
Nicotiana tabacum | ? | - |
? | |
S-adenosyl-L-methionine + DNA | NtDRM1 is a de novo cytosine methyltransferase which actively excludes CpG substrate | Nicotiana tabacum | S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | - |
? | |
S-adenosyl-L-methionine + DNA | poly(dI-dC) is a better substrate than poly(dG-dC), methylation activity toward unmethylated synthetic as well as native DNA. Activity with hemimethylated DNA is lower than that for unmethylated substrates. Methylation of about 70% of the cytosines in methylatable CpNpN and CpNpG sequences but only 10% in CpG. The enzyme non-selectively methylates any cytosines except in CpG, regardless of the adjacent nucleotide at both 5' and 3' ends | Nicotiana tabacum | S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | - |
? | |
S-adenosyl-L-methionine + hemimethylated CpG | - |
Nicotiana tabacum | ? | - |
? | |
S-adenosyl-L-methionine + poly(dG-dC) | - |
Nicotiana tabacum | ? | - |
? | |
S-adenosyl-L-methionine + poly(dI-dC) | - |
Nicotiana tabacum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Nt-DRM1 | - |
Nicotiana tabacum |