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Literature summary for 2.1.1.37 extracted from

  • Pradhan, S.; Bacolla, A.; Wells, R.D.; Roberts, R.J.
    Recombinant human DNA (cytosine-5) methyltransferase. I. Expression, purification, and comparison of DE Novo and maintenance methylation (1999), J. Biol. Chem., 274, 33002-33010.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of the enzyme by using a protein splicing fusion partner in a baculovirus expression vector Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Homo sapiens
0.0005
-
poly(dI-dC)*poly(dI-dC)
-
Homo sapiens
0.0072
-
S-adenosyl-L-methionine
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Storage Stability

Storage Stability Organism
-80°C, little loss of activity after 4 weeks, significant loss of activity after 6 weeks Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + DNA
-
Homo sapiens S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
?
S-adenosyl-L-methionine + DNA poly(dI-dC)*poly(dI-dC) and unmethylated and hemimethylated 36-mer and 75-mer oligonucleotides. The preference of the enzyme for hemimethylated, over unmethylated DNA is 7-21fold. Poly(dI-dC)‘poly(dI-dC) Homo sapiens S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
?
S-adenosyl-L-methionine + poly(dI-dC)*poly(dI-dC)
-
Homo sapiens ?
-
?
S-adenosyl-L-methionine + poly-(dI-dC)/poly(dI-dC)
-
Homo sapiens ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Homo sapiens
0.0511
-
poly(dI-dC)poly(dI-dC)
-
Homo sapiens