Literature summary for 2.1.1.35 extracted from

Alian, A.; Lee, T.T.; Griner, S.L.; Stroud, R.M.; Finer-Moore, J.
Structure of a TrmA-RNA complex: A consensus RNA fold contributes to substrate selectivity and catalysis in m5U methyltransferases (2008), Proc. Natl. Acad. Sci. USA, 105, 6876-6881.

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Cloned(Commentary)

Cloned (Comment)	Organism
into the pET28b expression vector	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
the structure of Escherichia coli E358Q 5-methyluridine 54 tRNA methyltransferase, in a covalent complex with a 19 nt T arm analog, is solved to 2.4 A resolution	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
E358Q	mutation of the catalytic base Glu-358 to Gln arrests catalysis and allows isolation of the covalent protein-RNA complex for crystallization	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + tRNA containing uridine at position 54	Escherichia coli	-	S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P23003	-	-

Purification (Commentary)

Purification (Comment)	Organism
the His6 tag protein is purified using a Talon column, the tag is cleaved by thrombin, thrombin is removed by passage over a benzamidine column	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + tRNA containing uridine at position 54	-	Escherichia coli	S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54	-	?

Synonyms

Synonyms	Comment	Organism
5-methyluridine 54 tRNA methyltransferase	-	Escherichia coli
m5U 54 tRNA methyltransferase	-	Escherichia coli
m5U 54 tRNA MTase	-	Escherichia coli
m5U methyltransferase	-	Escherichia coli
RUMT	-	Escherichia coli
TrmA	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Escherichia coli

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Release 2023.1 (January 2023)