Cloned (Comment) | Organism |
---|---|
into the pET28b expression vector | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
the structure of Escherichia coli E358Q 5-methyluridine 54 tRNA methyltransferase, in a covalent complex with a 19 nt T arm analog, is solved to 2.4 A resolution | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E358Q | mutation of the catalytic base Glu-358 to Gln arrests catalysis and allows isolation of the covalent protein-RNA complex for crystallization | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + tRNA containing uridine at position 54 | Escherichia coli | - |
S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P23003 | - |
- |
Purification (Comment) | Organism |
---|---|
the His6 tag protein is purified using a Talon column, the tag is cleaved by thrombin, thrombin is removed by passage over a benzamidine column | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + tRNA containing uridine at position 54 | - |
Escherichia coli | S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5-methyluridine 54 tRNA methyltransferase | - |
Escherichia coli |
m5U 54 tRNA methyltransferase | - |
Escherichia coli |
m5U 54 tRNA MTase | - |
Escherichia coli |
m5U methyltransferase | - |
Escherichia coli |
RUMT | - |
Escherichia coli |
TrmA | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |