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Literature summary for 2.1.1.35 extracted from
- High-level expression and rapid purification of tRNA (m5U54)-methyltransferase (1994), Protein Expr. Purif., 5, 149-152.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| trmA gene encoding for RUMT is cloned into a pET vector and transformed into Escherichia coli BL21 (DE3) cells, extremely high-level expression system | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-Fluorouracil substituted tRNA | first-order inactivation in the presence of S-adenosyl-L-methionine, potent mechanism-based irreversible inhibition, formation of a stable covalent complex containing enzyme, FUra-tRNA and the methyl group of AdoMet | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
x * 42000, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli GB-1-5-39 / pTN102 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| rapid purification scheme | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00165 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + uridine54 in tRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | methylates 5-carbon of U in position 54 of tRNA, product is ribothymidine, which is invariant in the TPsiC loop | ? |  |
| S-adenosyl-L-methionine + uridine54 in tRNA | - |
Escherichia coli GB-1-5-39 / pTN102 | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | methylates 5-carbon of U in position 54 of tRNA, product is ribothymidine, which is invariant in the TPsiC loop | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay at room temperature | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Escherichia coli | |
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