Cloned (Comment) | Organism |
---|---|
trmA gene encodes enzyme, gene is essential for the organism | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-Fluorouracil substituted tRNA | potent inhibitor | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uridine54 in tRNA | Escherichia coli | enzyme-tRNA complexes may exist as dihydrouridine intermediates and may serve as reservoirs of RUMT-tRNA complexes primed for methylation | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + uracil54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil54 in tRNA | steric course of methyl transfer | Escherichia coli | |
S-adenosyl-L-methionine + uracil54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil54 in tRNA | single SN2 displacement mechanism | Escherichia coli | |
S-adenosyl-L-methionine + uracil54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil54 in tRNA | detailed chemical and enzymatic mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | detailed mechanism, Cys-324 is the nucleophilic catalyst of enzyme, involves SN2 attack of C5 of U54 on the methyl group of AdoMet | Escherichia coli | additional information | - |
? | |
additional information | up to 50% of enzyme is covalently bound to various tRNA species, including several tRNAs at the 3 end of 16S rRNA | Escherichia coli | additional information | - |
? | |
additional information | enzyme also catalyzes an S-adenosyl-L-methionine-independent exchange reaction between [5-3H]-Urd-labeled substrate tRNA and protons of water at 1% of the normal methylation reaction rate, mechanism | Escherichia coli | additional information | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptor: methyl-deficient tRNA from E. coli | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | methylates 5-carbon of U in position 54 of tRNA, product is ribothymidine, which is invariant in the TPsiC loop | ? | |
S-adenosyl-L-methionine + uridine54 in tRNA | tRNA recognition | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | methylates 5-carbon of U in position 54 of tRNA, product is ribothymidine, which is invariant in the TPsiC loop | ? | |
S-adenosyl-L-methionine + uridine54 in tRNA | enzyme-tRNA complexes may exist as dihydrouridine intermediates and may serve as reservoirs of RUMT-tRNA complexes primed for methylation | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | uses S-adenosyl-L-methionine as cofactor | Escherichia coli |