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Literature summary for 2.1.1.35 extracted from

  • Gustafsson, C.; Björk, G.R.
    The tRNA-(m5U54)-methyltransferase of Escherichia coli is present in two forms in vivo, one of which is present as bound to tRNA and to a 3-end fragment of 16S rRNA (1993), J. Biol. Chem., 268, 1326-1331.
    View publication on PubMed
Search for more literature for 2.1.1.35

Cloned(Commentary)

Cloned (Comment) Organism
trmA gene encodes enzyme, gene is essential for the organism Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 native enzyme form, sucrose density gradient centrifugation Escherichia coli
54000
-
 TrmA/RNA complex, sucrose density gradient centrifugation Escherichia coli
62000
-
 x * 54000, first TrmA/RNA-complex, x * 62000, second TrmA/RNA-complex, x * 42000, native enzyme form, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + uridine54 in tRNA Escherichia coli in vivo present both as 42 kDa polypeptide and as covalent TrmA/RNA complex, to equal amounts, both with methylating activity, in vivo the m5U54 modification confers a small selective growth advantage, two different catalytic activities: synthesis of m5U54 and an unknown essential function, may be associated with binding RNA, either during a maturation process of the rRNA and/or tRNA or as a regulatory device during the assembly and maturation of the translational apparatus such as an RNA chaperone S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?
S-adenosyl-L-methionine + uridine54 in tRNA Escherichia coli 1100 in vivo present both as 42 kDa polypeptide and as covalent TrmA/RNA complex, to equal amounts, both with methylating activity, in vivo the m5U54 modification confers a small selective growth advantage, two different catalytic activities: synthesis of m5U54 and an unknown essential function, may be associated with binding RNA, either during a maturation process of the rRNA and/or tRNA or as a regulatory device during the assembly and maturation of the translational apparatus such as an RNA chaperone S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli
-
 3 enzyme forms: native protein and 2 different TrmA/RNA complexes
-
Escherichia coli 1100
-
-
-

Purification (Commentary)

Purification (Comment) Organism
3 enzyme forms Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + uracil54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil54 in tRNA catalytic mechanism Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme is present as 42 kDa native protein or as 2 different covalent TrmA/RNA complexes, with TrmA as a 54 kDa and a 62 kDa polypeptide, respectively, RNA is a subset of undermodified tRNA species as well as the 3Â’ terminus of 16S rRNA, RNA does not affect enzyme activity Escherichia coli additional information
-
 ?
additional information enzyme is present as 42 kDa native protein or as 2 different covalent TrmA/RNA complexes, with TrmA as a 54 kDa and a 62 kDa polypeptide, respectively, RNA is a subset of undermodified tRNA species as well as the 3Â’ terminus of 16S rRNA, RNA does not affect enzyme activity Escherichia coli 1100 additional information
-
 ?
S-adenosyl-L-methionine + uridine54 in tRNA enzyme transfers methyl group to uridine in position 54 of the TPsi-loop of all E. coli tRNA species, requires the TPsi-arm and, most specifically, the bases U54-U55-C56-Pu57-A58 for tRNA recognition Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA formation of 5-methyluridine ?
S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptor: tRNA from E. coli Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA formation of 5-methyluridine ?
S-adenosyl-L-methionine + uridine54 in tRNA in vivo present both as 42 kDa polypeptide and as covalent TrmA/RNA complex, to equal amounts, both with methylating activity, in vivo the m5U54 modification confers a small selective growth advantage, two different catalytic activities: synthesis of m5U54 and an unknown essential function, may be associated with binding RNA, either during a maturation process of the rRNA and/or tRNA or as a regulatory device during the assembly and maturation of the translational apparatus such as an RNA chaperone Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?
S-adenosyl-L-methionine + uridine54 in tRNA enzyme transfers methyl group to uridine in position 54 of the TPsi-loop of all E. coli tRNA species, requires the TPsi-arm and, most specifically, the bases U54-U55-C56-Pu57-A58 for tRNA recognition Escherichia coli 1100 S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA formation of 5-methyluridine ?
S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptor: tRNA from E. coli Escherichia coli 1100 S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA formation of 5-methyluridine ?
S-adenosyl-L-methionine + uridine54 in tRNA in vivo present both as 42 kDa polypeptide and as covalent TrmA/RNA complex, to equal amounts, both with methylating activity, in vivo the m5U54 modification confers a small selective growth advantage, two different catalytic activities: synthesis of m5U54 and an unknown essential function, may be associated with binding RNA, either during a maturation process of the rRNA and/or tRNA or as a regulatory device during the assembly and maturation of the translational apparatus such as an RNA chaperone Escherichia coli 1100 S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?

Subunits

Subunits Comment Organism
? x * 54000, first TrmA/RNA-complex, x * 62000, second TrmA/RNA-complex, x * 42000, native enzyme form, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Escherichia coli