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Literature summary for 2.1.1.33 extracted from
- Production of yeast tRNA (m(7)G46) methyltransferase (Trm8-Trm82 complex) in a wheat germ cell-free translation system (2008), J. Biotechnol., 133, 453-460.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in a wheat germ cell-free translation system, active Trm8-Trm82 heterodimer is only synthesized under conditions, in which both Trm8 and Trm82 mRNAs are cotranslated | Saccharomyces cerevisiae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
1 * 33000 (subunit Trm8) + 1 * 50000 (subunit Trm82), association of the Trm8 and Trm82 subunits is translationally controlled in living cells, SDS-PAGE | Saccharomyces cerevisiae |
| 50000 | - |
1 * 33000 (subunit Trm8) + 1 * 50000 (subunit Trm82), association of the Trm8 and Trm82 subunits is translationally controlled in living cells, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + guanine46 in tRNA | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, Sephadex G-25 gel filtration, ammonium sulfate precipitation, and DE52 column chromatography | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + guanine46 in tRNA | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 33000 (subunit Trm8) + 1 * 50000 (subunit Trm82), association of the Trm8 and Trm82 subunits is translationally controlled in living cells, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
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