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Literature summary for 2.1.1.325 extracted from

  • Van Ekert, E.; Shatters, R.G.; Rouge, P.; Powell, C.A.; Smagghe, G.; Borovsky, D.
    Cloning and expressing a highly functional and substrate specific farnesoic acid O-methyltransferase from the Asian citrus psyllid (Diaphorina citri Kuwayama) (2015), FEBS Open Bio, 5, 264-275.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.325

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli One Shot BL21 (DE3) cells Diaphorina citri

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ complete inhibition at 1 mM Diaphorina citri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00002
-
 homofarnesoate at pH 7.9 and 25°C Diaphorina citri
0.00046
-
 (2E,6E)-farnesoate at pH 7.9 and 25°C Diaphorina citri
0.00253
-
 juvenile hormone III acid at pH 7.9 and 25°C Diaphorina citri
0.00458
-
 (2E,6Z)-10cis-juvenile hormone I acid at pH 7.9 and 25°C Diaphorina citri
0.00565
-
 (2Z,6E)-10cis-juvenile hormone I acid at pH 7.9 and 25°C Diaphorina citri

Metals/Ions

Metals/Ions Comment Organism Structure
additional information does not require Ca2+, Mg2+ or Zn2+ Diaphorina citri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 x * 31000, SDS-PAGE Diaphorina citri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + (2E,6E)-farnesoate Diaphorina citri preferred substrate S-adenosyl-L-homocysteine + methyl (2E,6E)-farnesoate
-
 ?

Organism

Organism UniProt Comment Textmining
Diaphorina citri A0A075W3Z0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Diaphorina citri

Source Tissue

Source Tissue Comment Organism Textmining
corpora allata
-
 Diaphorina citri
-
male accessory gland
-
 Diaphorina citri
-
ovary
-
 Diaphorina citri
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + (2E,6E)-farnesoate preferred substrate Diaphorina citri S-adenosyl-L-homocysteine + methyl (2E,6E)-farnesoate
-
 ?
S-adenosyl-L-methionine + (2E,6Z)-10cis-juvenile hormone I acid
-
 Diaphorina citri S-adenosyl-L-homocysteine + juvenile hormone I
-
 ?
S-adenosyl-L-methionine + (2Z,6E)-10cis-juvenile hormone I acid
-
 Diaphorina citri S-adenosyl-L-homocysteine + juvenile hormone I
-
 ?
S-adenosyl-L-methionine + homofarnesoate
-
 Diaphorina citri S-adenosyl-L-homocysteine + methylhomofarnesoate
-
 ?
S-adenosyl-L-methionine + juvenile hormone III acid
-
 Diaphorina citri S-adenosyl-L-homocysteine + juvenile hormone III
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Diaphorina citri

Synonyms

Synonyms Comment Organism
FA-o-methyl transferase
-
 Diaphorina citri
farnesoic acid O-methyltransferase
-
 Diaphorina citri
fmtD
-
 Diaphorina citri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000003
-
 (2E,6Z)-10cis-juvenile hormone I acid at pH 7.9 and 25°C Diaphorina citri
0.000008
-
 (2Z,6E)-10cis-juvenile hormone I acid at pH 7.9 and 25°C Diaphorina citri
0.000013
-
 juvenile hormone III acid at pH 7.9 and 25°C Diaphorina citri
0.00022
-
 homofarnesoate at pH 7.9 and 25°C Diaphorina citri
0.00075
-
 (2E,6E)-farnesoate at pH 7.9 and 25°C Diaphorina citri

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00066
-
 (2E,6Z)-10cis-juvenile hormone I acid at pH 7.9 and 25°C Diaphorina citri
0.00514
-
 juvenile hormone III acid at pH 7.9 and 25°C Diaphorina citri
0.1434
-
 (2Z,6E)-10cis-juvenile hormone I acid at pH 7.9 and 25°C Diaphorina citri
1.645
-
 (2E,6E)-farnesoate at pH 7.9 and 25°C Diaphorina citri
10.85
-
 homofarnesoate at pH 7.9 and 25°C Diaphorina citri