Literature summary for 2.1.1.302 extracted from

Ding, W.; Deng, W.; Tang, M.; Zhang, Q.; Tang, G.; Bi, Y.; Liu, W.
Biosynthesis of 3-methoxy-5-methyl naphthoic acid and its incorporation into the antitumor antibiotic azinomycin B (2010), Mol. Biosyst., 6, 1071-1081.

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Activating Compound

Activating Compound	Comment	Organism	Structure
EDTA	5 mM, 10% increase inactivity	Streptomyces sahachiroi

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Streptomyces albus or in Escherichia coli Bö21(DE3)	Streptomyces sahachiroi

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	5 mM, about 15% inhibition	Streptomyces sahachiroi
Fe2+	5 mM, about 20% inhibition	Streptomyces sahachiroi
Mg2+	5 mM, about 5% inhibition	Streptomyces sahachiroi
Mn2+	5 mM, about 15% inhibition	Streptomyces sahachiroi
Zn2+	5 mM, about 45% inhibition	Streptomyces sahachiroi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.061	-	S-adenosyl-L-methionine	pH 8.0, 25°C	Streptomyces sahachiroi
0.145	-	3-hydroxy-5-methylnaphthoate	pH 8.0, 25°C	Streptomyces sahachiroi

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + 3-hydroxy-5-methylnaphthoate	Streptomyces sahachiroi	the enzyme is involved in biosynthesis of 3-methoxy-5-methyl naphthoate a component of of the the antitumor antibiotic azinomycin B	S-adenosyl-L-homocysteine + 3-methoxy-5-methylnaphthoate	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces sahachiroi	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Streptomyces sahachiroi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + 3-hydroxy-5-methylnaphthoate	-	Streptomyces sahachiroi	S-adenosyl-L-homocysteine + 3-methoxy-5-methylnaphthoate	-	?
S-adenosyl-L-methionine + 3-hydroxy-5-methylnaphthoate	the enzyme is involved in biosynthesis of 3-methoxy-5-methyl naphthoate a component of of the the antitumor antibiotic azinomycin B	Streptomyces sahachiroi	S-adenosyl-L-homocysteine + 3-methoxy-5-methylnaphthoate	-	?

Synonyms

Synonyms	Comment	Organism
AziB2	-	Streptomyces sahachiroi

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Streptomyces sahachiroi

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0063	-	S-adenosyl-L-methionine	pH 8.0, 25°C	Streptomyces sahachiroi
0.0073	-	3-hydroxy-5-methylnaphthoate	pH 8.0, 25°C	Streptomyces sahachiroi

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Streptomyces sahachiroi
9.5	-	-	Streptomyces sahachiroi

pH Range

pH Minimum	pH Maximum	Comment	Organism
7.5	10	pH 7.5: about 50% of maximal activity, pH 10.0: about 55% of maximal activity	Streptomyces sahachiroi

General Information

General Information	Comment	Organism
physiological function	the enzyme is involved in biosynthesis of 3-methoxy-5-methyl naphthoate a component of of the the antitumor antibiotic azinomycin B	Streptomyces sahachiroi

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.05	-	3-hydroxy-5-methylnaphthoate	pH 8.0, 25°C	Streptomyces sahachiroi
0.1	-	S-adenosyl-L-methionine	pH 8.0, 25°C	Streptomyces sahachiroi

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Release 2023.1 (January 2023)