Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CapG | the hMTr2 MTase activity is dependent on the presence of capG | Homo sapiens | |
additional information | neither N7 methylation of the guanosine cap nor 2'-O-ribose methylation of the first transcribed nucleotide are required for hMTr2, but the presence of cap1 methylation increases hMTr2 activity | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene HMTR2 or FTSJD1, phylogenetic tree, functional overexpression of the cap2 MTase hMTr2 and of c-myc-tagged enzyme in MCF7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutants of the K-D-K triad active site residues exchanged for alanine are all cataltyically inactive | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
additional information | localization of hMTr2 assayed in situ by immunostaining of epitope-tagged protein | Homo sapiens | - |
- |
nucleus | - |
Homo sapiens | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | hMTr2 protein transfers a methyl group from S-adenosylmethionine to the 2'-O-ribose of the second nucleotide of messenger RNA and small nuclear RNA | ? | - |
? | |
S-adenosyl-L-methionine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-(ribonucleotide)-[mRNA] | Homo sapiens | - |
S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-(2'-O-methyl-ribonucleotide)-[mRNA] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8IYT2 | gene FTSJD1 or HMTR2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | hMTr2 protein transfers a methyl group from S-adenosylmethionine to the 2'-O-ribose of the second nucleotide of messenger RNA and small nuclear RNA | Homo sapiens | ? | - |
? | |
additional information | the recombinant enzyme produces a RNase T2-resistant RNA fragment, cap012, from cap01-RNA, but shows no activity with a purified BAP protein. Only a subset of labeled RNA molecules that has GpppG incorporated during transcription is a substrate for hMTr2. hMTr2 recognizes TMG-capped snRNAs in vitro | Homo sapiens | ? | - |
? | |
S-adenosyl-L-methionine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-(ribonucleotide)-[mRNA] | - |
Homo sapiens | S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-(2'-O-methyl-ribonucleotide)-[mRNA] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cap2 | - |
Homo sapiens |
cap2-MTase | - |
Homo sapiens |
hMTr2 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | the N-terminal RFM domain has a conserved S-adenosyl-L-methionine-binding site (motifs I-III) | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the Rossmann-fold MTase (RFM) family, hMTr1 and hMTr2 are paralogues forming a subfamily with higher eukaryotic and viral members, minimum evolution tree of homologues of known 2'-O-ribose mRNA cap MTases, overview | Homo sapiens |
additional information | FTSJD1, the candidate hMTr2, is composed of two RFM domains, sequence comparisons, and has a K-D-K active site. Each residue of the K-D-K triad is essential for the activity of the enzyme | Homo sapiens |
physiological function | the 5' cap of human messenger RNA consists of an inverted 7-methylguanosine linked to the first transcribed nucleotide by a unique 5'-5' triphosphate bond followed by 2'-O-ribose methylation of the first and often the second transcribed nucleotides, likely serving to modify efficiency of transcript processing, translation and stability. Cap2 methylates the ribose of the second transcribed nucleotide. Relationship to other cap-modifying enzymes, overview | Homo sapiens |