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Literature summary for 2.1.1.296 extracted from

  • Werner, M.; Purta, E.; Kaminska, K.; Cymerman, I.; Campbell, D.; Mittra, B.; Zamudio, J.; Sturm, N.; Jaworski, J.; Bujnicki, J.
    2'-O-ribose methylation of cap2 in human: function and evolution in a horizontally mobile family (2011), Nucleic Acids Res., 39, 4756-4768.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
CapG the hMTr2 MTase activity is dependent on the presence of capG Homo sapiens
additional information neither N7 methylation of the guanosine cap nor 2'-O-ribose methylation of the first transcribed nucleotide are required for hMTr2, but the presence of cap1 methylation increases hMTr2 activity Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene HMTR2 or FTSJD1, phylogenetic tree, functional overexpression of the cap2 MTase hMTr2 and of c-myc-tagged enzyme in MCF7 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information mutants of the K-D-K triad active site residues exchanged for alanine are all cataltyically inactive Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-
additional information localization of hMTr2 assayed in situ by immunostaining of epitope-tagged protein Homo sapiens
-
-
nucleus
-
Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens hMTr2 protein transfers a methyl group from S-adenosylmethionine to the 2'-O-ribose of the second nucleotide of messenger RNA and small nuclear RNA ?
-
?
S-adenosyl-L-methionine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-(ribonucleotide)-[mRNA] Homo sapiens
-
S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-(2'-O-methyl-ribonucleotide)-[mRNA]
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8IYT2 gene FTSJD1 or HMTR2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information hMTr2 protein transfers a methyl group from S-adenosylmethionine to the 2'-O-ribose of the second nucleotide of messenger RNA and small nuclear RNA Homo sapiens ?
-
?
additional information the recombinant enzyme produces a RNase T2-resistant RNA fragment, cap012, from cap01-RNA, but shows no activity with a purified BAP protein. Only a subset of labeled RNA molecules that has GpppG incorporated during transcription is a substrate for hMTr2. hMTr2 recognizes TMG-capped snRNAs in vitro Homo sapiens ?
-
?
S-adenosyl-L-methionine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-(ribonucleotide)-[mRNA]
-
Homo sapiens S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-(2'-O-methyl-ribonucleotide)-[mRNA]
-
?

Synonyms

Synonyms Comment Organism
cap2
-
Homo sapiens
cap2-MTase
-
Homo sapiens
hMTr2
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine the N-terminal RFM domain has a conserved S-adenosyl-L-methionine-binding site (motifs I-III) Homo sapiens

General Information

General Information Comment Organism
evolution the enzyme belongs to the Rossmann-fold MTase (RFM) family, hMTr1 and hMTr2 are paralogues forming a subfamily with higher eukaryotic and viral members, minimum evolution tree of homologues of known 2'-O-ribose mRNA cap MTases, overview Homo sapiens
additional information FTSJD1, the candidate hMTr2, is composed of two RFM domains, sequence comparisons, and has a K-D-K active site. Each residue of the K-D-K triad is essential for the activity of the enzyme Homo sapiens
physiological function the 5' cap of human messenger RNA consists of an inverted 7-methylguanosine linked to the first transcribed nucleotide by a unique 5'-5' triphosphate bond followed by 2'-O-ribose methylation of the first and often the second transcribed nucleotides, likely serving to modify efficiency of transcript processing, translation and stability. Cap2 methylates the ribose of the second transcribed nucleotide. Relationship to other cap-modifying enzymes, overview Homo sapiens