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Literature summary for 2.1.1.293 extracted from

  • Kato, N.; Suzuki, H.; Okumura, H.; Takahashi, S.; Osada, H.
    A point mutation in ftmD blocks the fumitremorgin biosynthetic pathway in Aspergillus fumigatus strain Af293 (2013), Biosci. Biotechnol. Biochem., 77, 1061-1067.
    View publication on PubMed
Search for more literature for 2.1.1.293

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Aspergillus fumigatus

Protein Variants

Protein Variants Comment Organism
R202L the mutant has 19.7times lower catalytic efficiency compared to the wild type enzyme Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
 S-adenosyl-L-methionine mutant enzyme R202L, at pH 6.8 and 15°C Aspergillus fumigatus
0.011
-
 S-adenosyl-L-methionine wild type enzyme, at pH 6.8 and 15°C Aspergillus fumigatus
0.021
-
 6-hydroxytryprostatin B wild type enzyme, at pH 6.8 and 15°C Aspergillus fumigatus
0.12
-
 6-hydroxytryprostatin B mutant enzyme R202L, at pH 6.8 and 15°C Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 2 * 64000, SDS-PAGE Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 6-hydroxytryprostatin B Aspergillus fumigatus
-
 S-adenosyl-L-homocysteine + tryprostatin A
-
 ?
S-adenosyl-L-methionine + 6-hydroxytryprostatin B Aspergillus fumigatus Af293
-
 S-adenosyl-L-homocysteine + tryprostatin A
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus B9WZX2
-
-
Aspergillus fumigatus Af293 B9WZX2
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon-Co2+ column chromatography Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 6-hydroxytryprostatin B
-
 Aspergillus fumigatus S-adenosyl-L-homocysteine + tryprostatin A
-
 ?
S-adenosyl-L-methionine + 6-hydroxytryprostatin B
-
 Aspergillus fumigatus Af293 S-adenosyl-L-homocysteine + tryprostatin A
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 64000, SDS-PAGE Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
ftmD
-
 Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
-
 Aspergillus fumigatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 40 the wild type enzyme shows 100% activity at 10°C, about 95% activity at 20°C, about 75% activity at 30°C, about 35% activity at 35°C, and no activity at 40°C Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 S-adenosyl-L-methionine wild type enzyme, at pH 6.8 and 15°C Aspergillus fumigatus
6.9
-
 S-adenosyl-L-methionine mutant enzyme R202L, at pH 6.8 and 15°C Aspergillus fumigatus
8.2
-
 6-hydroxytryprostatin B mutant enzyme R202L, at pH 6.8 and 15°C Aspergillus fumigatus
27
-
 6-hydroxytryprostatin B wild type enzyme, at pH 6.8 and 15°C Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Aspergillus fumigatus

pH Range

pH Minimum pH Maximum Comment Organism
6 7 the wild type enzyme shows about 42% activity at pH 6.0, about 78% activity at pH 6.5, and about 90% activity at pH 7.0 Aspergillus fumigatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
66
-
 6-hydroxytryprostatin B mutant enzyme R202L, at pH 6.8 and 15°C Aspergillus fumigatus
1300
-
 6-hydroxytryprostatin B wild type enzyme, at pH 6.8 and 15°C Aspergillus fumigatus
1500
-
 S-adenosyl-L-methionine mutant enzyme R202L, at pH 6.8 and 15°C Aspergillus fumigatus
2500
-
 S-adenosyl-L-methionine wild type enzyme, at pH 6.8 and 15°C Aspergillus fumigatus