Cloned (Comment) | Organism |
---|---|
the recombinant protein is then expressed in Spodoptera frugiperda Sf9 cells in a baculovirus expression vector | Papaver somniferum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
isovanillic acid | pH 8.0, 35°C | Papaver somniferum | |
0.016 | - |
(S)-reticuline | pH 8.0, 35°C | Papaver somniferum | |
0.017 | - |
guaiacol | pH 8.0, 35°C | Papaver somniferum | |
0.017 | - |
(R)-reticuline | pH 8.0, 35°C | Papaver somniferum | |
0.15 | - |
S-adenosyl-L-methionine | pH 8.0, 35°C, cosubstrate isovanillic acid | Papaver somniferum | |
0.31 | - |
S-adenosyl-L-methionine | pH 8.0, 35°C, cosubstrate guaiacol or (R)-reticuline | Papaver somniferum | |
0.36 | - |
S-adenosyl-L-methionine | pH 8.0, 35°C, cosubstrate (S)-reticuline | Papaver somniferum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39841 | - |
2 * 39841, calculated from sequence | Papaver somniferum |
43000 | - |
2 * 43000, SDS-PAGE | Papaver somniferum |
85000 | - |
gel filtration | Papaver somniferum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + (R)-reticuline | Papaver somniferum | the enzyme is involved in tetrahydrobenzylisoquinoline biosynthesis | S-adenosyl-L-homocysteine + (R)-laudanine | - |
? | |
S-adenosyl-L-methionine + (S)-reticuline | Papaver somniferum | the enzyme is involved in tetrahydrobenzylisoquinoline biosynthesis | S-adenosyl-L-homocysteine + (S)-laudanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Papaver somniferum | Q6WUC2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Papaver somniferum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + (S)-reticuline = S-adenosyl-L-homocysteine + (S)-laudanine | (1) | Papaver somniferum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | (R)-protosinomenine and (R,S)-isoorientaline are O-methylated at C-6. The ration of kcat/Km for C-7 O-methylation compared to C6-O-methylation is 3.8:1 | Papaver somniferum | ? | - |
? | |
S-adenosyl-L-methionine + (R)-reticuline | the enzyme is involved in tetrahydrobenzylisoquinoline biosynthesis | Papaver somniferum | S-adenosyl-L-homocysteine + (R)-laudanine | - |
? | |
S-adenosyl-L-methionine + (R)-reticuline | the enzyme methylates (S)- and (R)-reticuline with equal efficiency | Papaver somniferum | S-adenosyl-L-homocysteine + (R)-laudanine | - |
? | |
S-adenosyl-L-methionine + (R,S)-orientaline | - |
Papaver somniferum | ? | - |
? | |
S-adenosyl-L-methionine + (S)-reticuline | the enzyme is involved in tetrahydrobenzylisoquinoline biosynthesis | Papaver somniferum | S-adenosyl-L-homocysteine + (S)-laudanine | - |
? | |
S-adenosyl-L-methionine + (S)-reticuline | the enzyme methylates (S)- and (R)-reticuline with equal efficiency | Papaver somniferum | S-adenosyl-L-homocysteine + (S)-laudanine | - |
? | |
S-adenosyl-L-methionine + guaiacol | - |
Papaver somniferum | ? | - |
? | |
S-adenosyl-L-methionine + isovanillic acid | - |
Papaver somniferum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000, SDS-PAGE | Papaver somniferum |
dimer | 2 * 39841, calculated from sequence | Papaver somniferum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
methylation of guaiacol, (R)-reticuline and (S)-reticuline | Papaver somniferum |
37 | 41 | methylation of (R,S)-isoorientaline and isovanillic acid | Papaver somniferum |
39 | - |
methylation of (R)-protosinomenine | Papaver somniferum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
isovanillic acid | pH 8.0, 35°C | Papaver somniferum | |
0.07 | - |
(S)-reticuline | pH 8.0, 35°C | Papaver somniferum | |
0.07 | - |
(R)-reticuline | pH 8.0, 35°C | Papaver somniferum | |
0.1 | - |
guaiacol | pH 8.0, 35°C | Papaver somniferum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | methylation of (R,S)-isoorientaline | Papaver somniferum |
7.5 | - |
isovanillic acid | Papaver somniferum |
8 | - |
methylation of guaiacol, (R)-reticuline and (S)-reticuline | Papaver somniferum |
9 | - |
methylation of (R)-protosinomenine | Papaver somniferum |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in in tetrahydrobenzylisoquinoline biosynthesis | Papaver somniferum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
(R)-reticuline | pH 8.0, 35°C | Papaver somniferum | |
4.5 | - |
(S)-reticuline | pH 8.0, 35°C | Papaver somniferum | |
5.9 | - |
guaiacol | pH 8.0, 35°C | Papaver somniferum |