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Literature summary for 2.1.1.279 extracted from

  • Koeduka, T.; Baiga, T.J.; Noel, J.P.; Pichersky, E.
    Biosynthesis of t-anethole in anise: characterization of t-anol/isoeugenol synthase and an O-methyltransferase specific for a C7-C8 propenyl side chain (2009), Plant Physiol., 149, 384-394.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.279

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pimpinella anisum

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 2.5 mM, 97% inhibition Pimpinella anisum
Fe2+ 2.5 mM, 47% inhibition Pimpinella anisum
Mn2+ 2.5 mM, 35% inhibition Pimpinella anisum
additional information no inhibitory effect on the activity is observed by incubation with 2.5 mM K+, Na+, Ca2+, and Mg2+ Pimpinella anisum
Zn2+ 2.5 mM, 97% inhibition Pimpinella anisum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0193
-
 isoeugenol pH 7.5, 25°C Pimpinella anisum
0.0545
-
 S-adenosyl-L-methionine pH 7.5, 25°C Pimpinella anisum

Organism

Organism UniProt Comment Textmining
Pimpinella anisum B8RCD3
-
-

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + isoeugenol = S-adenosyl-L-homocysteine + isomethyleugenol (2) Pimpinella anisum
a
S-adenosyl-L-methionine + trans-anol = S-adenosyl-L-homocysteine + trans-anethole (1) Pimpinella anisum
a

Source Tissue

Source Tissue Comment Organism Textmining
bud
-
 Pimpinella anisum
-
flower
-
 Pimpinella anisum
-
fruit the gene encoding AIMT1 is expressed throughout the plant and the transcript level is highest in developing fruits Pimpinella anisum
-
leaf young, very low activity in mature leaves Pimpinella anisum
-
root
-
 Pimpinella anisum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme does not have activity with eugenol or chavicol Pimpinella anisum ?
-
 ?
S-adenosyl-L-methionine + shows a 10fold preference for isoeugenol over eugenol The enzyme is highly specific for substrates in which the double bond in the propenyl side chain is located between C7 and C8 Pimpinella anisum S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + chavicol the enzyme shows a 10fold preference for t-anol over chavicol. The enzyme is highly specific for substrates in which the double bond in the propenyl side chain is located between C7 and C8 Pimpinella anisum S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + isoeugenol the enzyme prefers isoeugenol to t-anol by a factor of 2. The enzyme is highly specific for substrates in which the double bond in the propenyl side chain is located between C7 and C8 Pimpinella anisum S-adenosyl-L-homocysteine + isomethyleugenol
-
 ?
S-adenosyl-L-methionine + trans-anol trans-anol i.e. 4-[(1E)-prop-1-en-1-yl]phenol. The enzyme prefers isoeugenol to t-anol by a factor of 2. It shows a 10fold preference for t-anol over chavicol. The enzyme is highly specific for substrates in which the double bond in the propenyl side chain is located between C7 and C8 Pimpinella anisum S-adenosyl-L-homocysteine + trans-anethole trans-anethole i.e. 1-methoxy-4-[(1E)-prop-1-en-1-yl]benzene ?

Synonyms

Synonyms Comment Organism
AIMT1
-
 Pimpinella anisum
S-adenosyl-L-methionine:t-anol/isoeugenol O-methyltransferase
-
 Pimpinella anisum
t-anol O-methyltransferase
-
 Pimpinella anisum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 37 30 min, stable Pimpinella anisum
50
-
 30 min, 12% loss of activity Pimpinella anisum
65
-
 30 min, less than 5% of activity remaining Pimpinella anisum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.015
-
 isoeugenol pH 7.5, 25°C Pimpinella anisum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 Tris buffer Pimpinella anisum

pH Range

pH Minimum pH Maximum Comment Organism
6 9 active in the range Pimpinella anisum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.78
-
 isoeugenol pH 7.5, temperature not specified in the publication Pimpinella anisum