Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) CodonPlus cells | Populus trichocarpa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | complete inhibition at 5 mM | Populus trichocarpa | |
Fe2+ | nearly complete inhibition at 5 mM | Populus trichocarpa | |
Mg2+ | about 80% residual activity at 5 mM | Populus trichocarpa | |
Mn2+ | about 50% residual activity at 5 mM | Populus trichocarpa | |
Zn2+ | nearly complete inhibition at 5 mM | Populus trichocarpa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0231 | - |
S-adenosyl-L-methionine | at pH 7.5 and 25°C | Populus trichocarpa | |
0.0304 | - |
(indol-3-yl)acetate | at pH 7.5 and 25°C | Populus trichocarpa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | mild activation at 5 mM | Populus trichocarpa | |
K+ | about 225% activity at 5 mM | Populus trichocarpa | |
Na+ | mild activation at 5 mM | Populus trichocarpa | |
NH4+ | mild activation at 5 mM | Populus trichocarpa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Populus trichocarpa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + (indol-3-yl)acetate | Populus trichocarpa | highest activity | S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus trichocarpa | - |
clone Nisqually-1 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column agarose chromatography | Populus trichocarpa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | moderate expression in young leaves, no expression in old leaves | Populus trichocarpa | - |
root | low level of expression | Populus trichocarpa | - |
stem | highest level of expression | Populus trichocarpa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with salicylic acid, benzoic acid, jasmonic acid, and farnesoic acid | Populus trichocarpa | ? | - |
? | |
S-adenosyl-L-methionine + (indol-3-yl)acetate | highest activity | Populus trichocarpa | S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate | - |
? | |
S-adenosyl-L-methionine + (indol-3-yl)butyrate | 11% activity compared to (indol-3-yl)acetate | Populus trichocarpa | S-adenosyl-L-homocysteine + methyl (indol-3-yl)butyrate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Populus trichocarpa |
Synonyms | Comment | Organism |
---|---|---|
IAMT | - |
Populus trichocarpa |
indole-3-acetic acid methyltransferase | - |
Populus trichocarpa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Populus trichocarpa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 40 | activity at 4°C and 40°C is about 40% of the maximal activity | Populus trichocarpa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Populus trichocarpa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.7 | 9 | at pH 6.5, the enzyme has about 34% of its maximal activity, whereas at pH 9.0, the enzyme displays about 40% of the maximal activity | Populus trichocarpa |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in development of root and leaf in poplar and plays a role in wood formation | Populus trichocarpa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.782 | - |
S-adenosyl-L-methionine | at pH 7.5 and 25°C | Populus trichocarpa |