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Literature summary for 2.1.1.273 extracted from

  • Negre, F.; Kolosova, N.; Knoll, J.; Kish, C.M.; Dudareva, N.
    Novel S-adenosyl-L-methionine:salicylic acid carboxyl methyltransferase, an enzyme responsible for biosynthesis of methyl salicylate and methyl benzoate, is not involved in floral scent production in snapdragon flowers (2002), Arch. Biochem. Biophys., 406, 261-270.
    View publication on PubMed
Search for more literature for 2.1.1.273

Cloned(Commentary)

Cloned (Comment) Organism
subcloned into expression vector pET-28a, containing an N-terminal polyhistidine 6-His-tag, and expressed in Escherichia coli Antirrhinum majus

General Stability

General Stability Organism
the enzyme is active in both Tris- and phosphate–citrate-based buffers, although its activity in phosphate-citrate buffer is about 20% lower than in Tris-buffer Antirrhinum majus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 5 mM, strong inhibitory effect with more than 95% inhibition Antirrhinum majus
Fe2+ 5 mM, strong inhibitory effect with more than 95% inhibition Antirrhinum majus
Mn2+ 5 mM, inhibits activity less than 30% Antirrhinum majus
additional information presence of 5 mM Mg2+ in the assay reaction and addition of K+, NH4+ and Ca2+ does not affect activity Antirrhinum majus
Na+ 5 mM, inhibits activity less than 30% Antirrhinum majus
Zn2+ 5 mM, inhibits activity less than 30% Antirrhinum majus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 S-adenosyl-L-methionine with benzoic acid, pH 7.5, 20°C Antirrhinum majus
1.72
-
 benzoic acid pH 7.5, 20°C Antirrhinum majus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 SDS-PAGE Antirrhinum majus
107000
-
 with a calibrated Sephacryl 200-HR column Antirrhinum majus

Organism

Organism UniProt Comment Textmining
Antirrhinum majus Q8H6N2 snapdragon
-

Purification (Commentary)

Purification (Comment) Organism
by nickel-based affinity chromatography Antirrhinum majus

Source Tissue

Source Tissue Comment Organism Textmining
additional information transcripts are undetectable in floral and leaf tissues by RNA-blot hybridization but low levels of gene expression detected with real-time RT-PCR Antirrhinum majus
-
petal 200fold higher levels of mRNA than in the pistil where lowest level of mRNA expression is observed Antirrhinum majus
-
sepal expression of mRNA about half the level in petal Antirrhinum majus
-
stamen expression of only 2fold higher than in pistil, where lowest level of mRNA expression is observed Antirrhinum majus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000054
-
 benzoic acid, pH 7.5, 20°C Antirrhinum majus

Storage Stability

Storage Stability Organism
the recombinant protein is catalytically stable for more than a year when stored at -80°C Antirrhinum majus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme is also active with salicylic acid resulting in methyl salicylate formation Antirrhinum majus ?
-
 ?
additional information no activity with 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, trans-cinnamic acid, p-coumaric acid, m-coumaric acid, o-coumaric acid and benzyl alcohol Antirrhinum majus ?
-
 ?
S-adenosyl-L-methionine + benzoic acid
-
 Antirrhinum majus S-adenosyl-L-homocysteine + methyl benzoate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 49000, SDS-PAGE Antirrhinum majus

Synonyms

Synonyms Comment Organism
BAMT
-
 Antirrhinum majus
benzoic acid carboxyl methyltransferase
-
 Antirrhinum majus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 enzyme is stable at temperatures up to 42°C for 30 min but 30 min incubation at 65°C leads to a 70% loss of activity Antirrhinum majus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0058
-
 benzoic acid pH 7.5, 20°C Antirrhinum majus
0.006
-
 S-adenosyl-L-methionine with benzoic acid, pH 7.5, 20°C Antirrhinum majus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Antirrhinum majus

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 at pH 4.0 and 9.5 the enzyme activity falls to about 20% of the maximal activity Antirrhinum majus
5 8 with 80–90% of maximum activity Antirrhinum majus