Any feedback?
Literature summary for 2.1.1.268 extracted from
- Actin-binding protein ABP140 is a methyltransferase for 3-methylcytidine at position 32 of tRNAs in Saccharomyces cerevisiae (2011), RNA, 17, 1111-1119.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of hexahistidine-tagged Abp140p in Saccharomces cerevisiae | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D466A | no formation of N3-methylcytosine32 | Saccharomyces cerevisiae |
| D547A | decreased activity | Saccharomyces cerevisiae |
| delD602-Q621 | no formation of N3-methylcytosine32 | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| actin filament | subcellular localization of ABP140 to actin filaments is not involved in tRNA modification | Saccharomyces cerevisiae | 5884 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 71486 | - |
2 * 71486, the homodimer is formed through covalent linkage(s) other than disulfide bond, calculated from sequence, SDS-PAGE | Saccharomyces cerevisiae |
| 71500 | - |
2 * 71500, the homodimer is formed through covalent linkage(s) other than disulfide bond, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + cytosine32 in tRNA1Ser | Saccharomyces cerevisiae | tRNASer1 = tRNASer(UGA). Because position 32 of tRNA has an important role in accurate codon recognition, N3-methylcytosine32 formation in tRNAThr1 or tRNASer1 might play a role in modulating codon recognition and translational efficiency | S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNA1Ser | - |
? |  |
| S-adenosyl-L-methionine + cytosine32 in tRNA1Thr | Saccharomyces cerevisiae | tRNAThr1 = tRNAThr(IGU). Because position 32 of tRNA has an important role in accurate codon recognition, N3-methylcytosine32 formation in tRNAThr1 or tRNASer1 might play a role in modulating codon recognition and translational efficiency | S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNA1Thr | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q6P1Q9 | - |
- |
| Saccharomyces cerevisiae | Q08641 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| S-adenosyl-L-methionine + cytosine32 in tRNASer = S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer | (2) | Saccharomyces cerevisiae | |
| S-adenosyl-L-methionine + cytosine32 in tRNAThr = S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNAThr | (1) | Saccharomyces cerevisiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + cytosine32 in tRNA1Ser | tRNASer1 = tRNASer(UGA). Because position 32 of tRNA has an important role in accurate codon recognition, N3-methylcytosine32 formation in tRNAThr1 or tRNASer1 might play a role in modulating codon recognition and translational efficiency | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNA1Ser | - |
? | |
| S-adenosyl-L-methionine + cytosine32 in tRNA1Ser | tRNAThr1 = tRNAThr(IGU). ABP140 is identified as the protein responsible for N3-methylcytosine32 formation in both tRNAThr1 and tRNASer1 by systematic reverse genetic approach combined with mass spectrometry (ribonucleome analysis). N3-Methylcytosine32 formation in tRNAThr1 can be reconstituted using recombinant Abp140p in the presence of S-adenosyl-L-methionine, whereas N3-methylcytosine32 does not form in tRNASer1 in vitro, indicating the absence of a factor(s) required for tRNASer1 N3-methylcytosine32 formation | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNA1Ser | - |
? | |
| S-adenosyl-L-methionine + cytosine32 in tRNA1Thr | tRNAThr1 = tRNAThr(IGU). Because position 32 of tRNA has an important role in accurate codon recognition, N3-methylcytosine32 formation in tRNAThr1 or tRNASer1 might play a role in modulating codon recognition and translational efficiency | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNA1Thr | - |
? | |
| S-adenosyl-L-methionine + cytosine32 in tRNA1Thr | tRNAThr1 = tRNAThr(IGU). ABP140 is identified as the protein responsible for N3-methylcytosine32 formation in both tRNAThr1 and tRNASer1 by systematic reverse genetic approach combined with mass spectrometry (ribonucleome analysis). N3-Methylcytosine32 formation in tRNAThr1 can be reconstituted using recombinant Abp140p in the presence of S-adenosyl-L-methionine, whereas N3-methylcytosine32 does not form in tRNASer1 in vitro, indicating the absence of a factor(s) required for tRNASer1 N3-methylcytosine32 formation | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNA1Thr | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 71486, the homodimer is formed through covalent linkage(s) other than disulfide bond, calculated from sequence, SDS-PAGE | Saccharomyces cerevisiae |
| dimer | 2 * 71500, the homodimer is formed through covalent linkage(s) other than disulfide bond, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ABP140 | - |
Saccharomyces cerevisiae |
| METTL2B | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Saccharomyces cerevisiae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | N3-methylcytosine32 modification is absent in strains that lack the entire ABP140 gene | Saccharomyces cerevisiae |
| malfunction | specific reduction of N3-methylcytosine formation in HeLa cells by siRNA-mediated knock down of METTL2B | Homo sapiens |
| physiological function | N3-methylcytosine32 formation in tRNAThr1 or tRNASer1 might play a role in modulating codon recognition and translational efficiency | Saccharomyces cerevisiae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
