Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00057 | - |
quercetin | pH 7.5, 30°C | Solanum lycopersicum | |
0.00074 | - |
luteolin | pH 7.5, 30°C | Solanum lycopersicum | |
0.0224 | - |
eriodictyol | pH 7.5, 30°C | Solanum lycopersicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
cultivar Micro-Tom | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
eriodictyol + S-adenosyl-L-methionine | 11% of the activity with quercetin | Solanum lycopersicum | homoeriodictyol + S-adenosyl-L-homocysteine | - |
? | |
laricitrin + S-adenosyl-L-methionine | 114% of the activity with quercetin | Solanum lycopersicum | syringetin + S-adenosyl-L-homocysteine | - |
? | |
luteolin + S-adenosyl-L-methionine | 42% of the activity with quercetin | Solanum lycopersicum | chrysoeriol + S-adenosyl-L-homocysteine | - |
? | |
additional information | enzyme is a regiospecific flavonoid 3'/5'-O-methyltransferase showing higher binding affinity and catalytic efficiency for quercetin and luteolin than for eriodictyol. No substrates: naringenin, apigenin, tricetin, kaempferol, daidzein, genistein | Solanum lycopersicum | ? | - |
? | |
quercetin + S-adenosyl-L-methionine | - |
Solanum lycopersicum | isorhamnetin + S-adenosyl-L-homocysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OMT3 | - |
Solanum lycopersicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
quercetin | pH 7.5, 30°C | Solanum lycopersicum | |
0.03 | - |
luteolin | pH 7.5, 30°C | Solanum lycopersicum | |
0.055 | - |
eriodictyol | pH 7.5, 30°C | Solanum lycopersicum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.45 | - |
eriodictyol | pH 7.5, 30°C | Solanum lycopersicum | |
40.5 | - |
luteolin | pH 7.5, 30°C | Solanum lycopersicum | |
45.6 | - |
quercetin | pH 7.5, 30°C | Solanum lycopersicum |