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Literature summary for 2.1.1.261 extracted from

  • Rigbers, O.; Li, S.M.
    Ergot alkaloid biosynthesis in Aspergillus fumigatus. Overproduction and biochemical characterization of a 4-dimethylallyltryptophan N-methyltransferase (2008), J. Biol. Chem., 283, 26859-26868.
    View publication on PubMed
Search for more literature for 2.1.1.261

Cloned(Commentary)

Cloned (Comment) Organism
gene fgaMT, DNA and amino acid sequence determination and analysis, overexpression of the soluble His6-tagged enzyme in Escherichia coli strain XL-1 blue MRF- Aspergillus fumigatus

Inhibitors

Inhibitors Comment Organism Structure
EDTA 13% inhibition at 5 mM Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 4-dimethylallyl-L-tryptophan pH 7.5, 37°C, recombinant enzyme Aspergillus fumigatus
2.4
-
 S-adenosyl-L-methionine pH 7.5, 37°C, recombinant enzyme Aspergillus fumigatus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not require metal ions Mg2+,Mn2+, Ca2+, K+, and Na+ for its enzymatic reaction Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38100
-
 2 * 40000, recombinant His6-tagged enzyme, SDS-PAGE, x * 39200, about, His6-tagged enzyme, sequence calculation, x * 38100, about, non-tagged enzyme, sequence calculation Aspergillus fumigatus
39200
-
 2 * 40000, recombinant His6-tagged enzyme, SDS-PAGE, x * 39200, about, His6-tagged enzyme, sequence calculation, x * 38100, about, non-tagged enzyme, sequence calculation Aspergillus fumigatus
40000
-
 2 * 40000, recombinant His6-tagged enzyme, SDS-PAGE, x * 39200, about, His6-tagged enzyme, sequence calculation, x * 38100, about, non-tagged enzyme, sequence calculation Aspergillus fumigatus
75000
-
 recombinant His6-tagged enzyme, gel filtration Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan Aspergillus fumigatus
-
 S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
 ?
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan Aspergillus fumigatus B5233
-
 S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus B6D5I7 gene fgaMT, ATCC 13073
-
Aspergillus fumigatus B5233 B6D5I7 gene fgaMT, ATCC 13073
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble His6-tagged enzyme in Escherichia coli strain XL-1 blue MRF- by nickel affinity chromatography and gel filtration to apparent homogeneity Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows a relatively high specificity toward the prenyl moiety at position C-4 of the indole ring, 4-dimethylallyltryptophan derivatives with modification at the indole ring are also accepted by FgaMT as substrates Aspergillus fumigatus ?
-
 ?
additional information the enzyme shows a relatively high specificity toward the prenyl moiety at position C-4 of the indole ring, 4-dimethylallyltryptophan derivatives with modification at the indole ring are also accepted by FgaMT as substrates Aspergillus fumigatus B5233 ?
-
 ?
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
-
 Aspergillus fumigatus S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
 ?
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan the enzyme shows a relatively high specificity toward the prenyl moiety at position C-4 of the indole ring Aspergillus fumigatus S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine product identificaation by NMR and mass spectrometry analysis ?
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
-
 Aspergillus fumigatus B5233 S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
 ?
S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan the enzyme shows a relatively high specificity toward the prenyl moiety at position C-4 of the indole ring Aspergillus fumigatus B5233 S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine product identificaation by NMR and mass spectrometry analysis ?

Subunits

Subunits Comment Organism
homodimer 2 * 40000, recombinant His6-tagged enzyme, SDS-PAGE, x * 39200, about, His6-tagged enzyme, sequence calculation, x * 38100, about, non-tagged enzyme, sequence calculation Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
fgaMT
-
 Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 S-adenosyl-L-methionine pH 7.5, 37°C, recombinant enzyme Aspergillus fumigatus
2
-
 4-dimethylallyl-L-tryptophan pH 7.5, 37°C, recombinant enzyme Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Aspergillus fumigatus

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Aspergillus fumigatus

General Information

General Information Comment Organism
metabolism FgaMT represents the second pathway-specific enzyme in the biosynthesis of ergot alkaloids Aspergillus fumigatus