Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Clostridioides difficile |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0178 | - |
Cobalamin | 25°C, pH 5.1 | Clostridioides difficile | |
0.06 | - |
Cobalamin | 25°C, pH 7.2 | Clostridioides difficile |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridioides difficile | - |
- |
- |
Clostridioides difficile 630 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cobalamin + 5-methyltetrahydrofolate | reaction is pH dependent with the best pH of around 5.0 | Clostridioides difficile | methylcobalamin + tetrahydrofolate | - |
? | |
cobalamin + 5-methyltetrahydrofolate | reaction is pH dependent with the best pH of around 5.0 | Clostridioides difficile 630 | methylcobalamin + tetrahydrofolate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.63 | - |
Cobalamin | 25°C, pH 5.1 | Clostridioides difficile | |
37.2 | - |
Cobalamin | 25°C, pH 7.2 | Clostridioides difficile |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Clostridioides difficile | calculated | - |
4.8 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
150 | - |
Cobalamin | 25°C, pH 5.1 | Clostridioides difficile | |
620 | - |
Cobalamin | 25°C, pH 7.2 | Clostridioides difficile |