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Literature summary for 2.1.1.258 extracted from

  • Zhao, S.; Roberts, D.; Ragsdale, S.
    Mechanistic studies of the methyltransferase from Clostridium thermoaceticum: Origin of the pH dependence of the methyl group transfer from methyltetrahydrofolate to the corrinoid/iron-sulfur protein (1995), Biochemistry, 34, 15075-15083.
    View publication on PubMed
Search for more literature for 2.1.1.258

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 5-methyltetrahydrofolate using hydroxocobalamin as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
0.01
-
 5-methyltetrahydrofolate using [Co(I) corrinoid Fe-S protein] as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
0.06
-
 [Co(I) corrinoid Fe-S protein] in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
0.1
-
 [methyl-Co(III) corrinoid Fe-S protein] Km above 0.1 mM, in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
0.2
-
 tetrahydrofolate Km above 0.2 mM, using [Co(I) corrinoid Fe-S protein] as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
0.341
-
 tetrahydrofolate using hydroxocobalamin as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
0.665
-
 methylcobalamin in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
2
-
 hydroxocobalamin in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica

Organism

Organism UniProt Comment Textmining
Moorella thermoacetica
-
 formerly Clostridium thermoaceticum
-

Purification (Commentary)

Purification (Comment) Organism
-
 Moorella thermoacetica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxocobalamin + 5-methyltetrahydrofolate
-
 Moorella thermoacetica methylcobalamin + tetrahydrofolate
-
 r
methylcobalamin + tetrahydrofolate
-
 Moorella thermoacetica hydroxocobalamin + 5-methyltetrahydrofolate
-
 r
[Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrofolate
-
 Moorella thermoacetica [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrofolate
-
 r
[methyl-Co(III) corrinoid Fe-S protein] + tetrahydrofolate
-
 Moorella thermoacetica [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrofolate
-
 r

Synonyms

Synonyms Comment Organism
methyltetrahydrofo1ate:corrinoid/iron-sulfur protein methyltransferase
-
 Moorella thermoacetica
MeTr
-
 Moorella thermoacetica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 [methyl-Co(III) corrinoid Fe-S protein] kcat below 0.02 s(-1), in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
1.1
-
 hydroxocobalamin in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
1.5
-
 5-methyltetrahydrofolate using hydroxocobalamin as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
2.2
-
 methylcobalamin in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
18
-
 [Co(I) corrinoid Fe-S protein] in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica

pI Value

Organism Comment pI Value Maximum pI Value
Moorella thermoacetica isoelectric focusing
-
 4.8

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1
-
 methylcobalamin in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
4.4
-
 tetrahydrofolate using hydroxocobalamin as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
5.2
-
 tetrahydrofolate using [Co(I) corrinoid Fe-S protein] as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
14.7
-
 hydroxocobalamin in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
17.1
-
 [methyl-Co(III) corrinoid Fe-S protein] in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
450
-
 5-methyltetrahydrofolate using hydroxocobalamin as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
1600
-
 [Co(I) corrinoid Fe-S protein] in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
10800
-
 5-methyltetrahydrofolate using [Co(I) corrinoid Fe-S protein] as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica