Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.258 extracted from

  • Seravalli, J.; Zhao, S.; Ragsdale, S.W.
    Mechanism of transfer of the methyl group from (6S)-methyltetrahydrofolate to the corrinoid/iron-sulfur protein catalyzed by the methyltransferase from Clostridium thermoaceticum: a key step in the Wood-Ljungdahl pathway of acetyl-CoA synthesis (1999), Biochemistry, 38, 5728-5735.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
tetrahydrofolate competitive inhibitor Moorella thermoacetica
[methyl-Co(III) corrinoid Fe-S protein] competitive inhibitor Moorella thermoacetica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
5-methyltetrahydrofolate in 50 mM MES, pH 5.1, at 25°C Moorella thermoacetica
0.012
-
[Co(I) corrinoid Fe-S protein] in 50 mM MES, pH 5.1, at 25°C Moorella thermoacetica

Organism

Organism UniProt Comment Textmining
Moorella thermoacetica
-
formerly Clostridium thermoaceticum
-

Purification (Commentary)

Purification (Comment) Organism
-
Moorella thermoacetica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrofolate
-
Moorella thermoacetica [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrofolate
-
r

Synonyms

Synonyms Comment Organism
methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase
-
Moorella thermoacetica
MeTr
-
Moorella thermoacetica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14.7
-
5-methyltetrahydrofolate in 50 mM MES, pH 5.1, at 25°C Moorella thermoacetica
14.7
-
[Co(I) corrinoid Fe-S protein] in 50 mM MES, pH 5.1, at 25°C Moorella thermoacetica

General Information

General Information Comment Organism
metabolism the enzyme performs a key step in the Wood-Ljungdahl pathway of acetyl-CoA synthesis Moorella thermoacetica