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Literature summary for 2.1.1.257 extracted from
- The archaeal COG1901/DUF358 SPOUT-methyltransferase members, together with pseudouridine synthase Pus10, catalyze the formation of 1-methylpseudouridine at position 54 of tRNA (2012), RNA, 18, 421-433.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Methanocaldococcus jannaschii TrmY requires C55 (or possibly U55) in the tRNA to produce m1PSI54 | Methanocaldococcus jannaschii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene trmY or HVO_1989 | Haloferax volcanii |
| gene VNG1980C, expression of the gene encoding COG1901 from Halobacterium sp. strain NRC-1 in Haloferax volcanii strain DHVO_1989 complements the m1PSI minus, enzyme-deficient phenotype of the mutant strain | Halobacterium sp. |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | expression of the gene VNG1980C encoding COG1901 from Halobacterium sp. strain NRC-1 in Haloferax volcanii strain DHVO_1989 complements the m1PSI minus, enzyme-deficient phenotype of the mutant strain | Haloferax volcanii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-homocysteine | - |
Halobacterium sp. |  |
| S-adenosyl-L-homocysteine | - |
Haloferax volcanii | |
| S-adenosyl-L-homocysteine | - |
Methanocaldococcus jannaschii | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + pseudouridine54 in tRNA | Haloferax volcanii | - |
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
| S-adenosyl-L-methionine + pseudouridine54 in tRNA | Halobacterium sp. | - |
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
| S-adenosyl-L-methionine + pseudouridine54 in tRNA | Methanocaldococcus jannaschii | - |
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
| S-adenosyl-L-methionine + pseudouridine54 in tRNA | Halobacterium sp. NRC-1 | - |
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halobacterium sp. | Q9HNR6 | gene VNG1980C | - |
| Halobacterium sp. NRC-1 | Q9HNR6 | gene VNG1980C | - |
| Haloferax volcanii | D4GTL8 | gene HVO_1989 or trmY | - |
| Methanocaldococcus jannaschii | Q59034 | gene Mj1640 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview | Haloferax volcanii | ? | - |
? | |
| additional information | enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview | Halobacterium sp. | ? | - |
? | |
| additional information | enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview | Methanocaldococcus jannaschii | ? | - |
? | |
| additional information | enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview | Halobacterium sp. NRC-1 | ? | - |
? | |
| S-adenosyl-L-methionine + pseudouridine54 in elongator tRNAMet | - |
Haloferax volcanii | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in elongator tRNAMet | - |
? | |
| S-adenosyl-L-methionine + pseudouridine54 in tRNA | - |
Haloferax volcanii | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
| S-adenosyl-L-methionine + pseudouridine54 in tRNA | - |
Halobacterium sp. | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
| S-adenosyl-L-methionine + pseudouridine54 in tRNA | - |
Methanocaldococcus jannaschii | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
| S-adenosyl-L-methionine + pseudouridine54 in tRNA | methylation of pseudouridine PSI at position 54 of tRNA, in vitro and in vivo reaction, producing m1PSI, in vitro pseudouridylated tRNATrp is used as a substrate | Methanocaldococcus jannaschii | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
| S-adenosyl-L-methionine + pseudouridine54 in tRNA | methylation of pseudouridine PSI at position 54 of tRNA, producing m1PSI, in vitro pseudouridylated tRNATrp is used as a substrate | Haloferax volcanii | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
| S-adenosyl-L-methionine + pseudouridine54 in tRNA | methylation of pseudouridine PSI at position 54 of tRNA, producing m1PSI, in vitro pseudouridylated tRNATrp is used as a substrate | Halobacterium sp. | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
| S-adenosyl-L-methionine + pseudouridine54 in tRNA | - |
Halobacterium sp. NRC-1 | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
| S-adenosyl-L-methionine + pseudouridine54 in tRNA | methylation of pseudouridine PSI at position 54 of tRNA, producing m1PSI, in vitro pseudouridylated tRNATrp is used as a substrate | Halobacterium sp. NRC-1 | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA | - |
? | |
| S-adenosyl-L-methionine + pseudouridine54 in tRNATrp | - |
Haloferax volcanii | S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNATrp | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| COG1901 | - |
Haloferax volcanii |
| COG1901 | - |
Halobacterium sp. |
| COG1901 | - |
Methanocaldococcus jannaschii |
| DUF358 | - |
Haloferax volcanii |
| DUF358 | - |
Halobacterium sp. |
| DUF358 | - |
Methanocaldococcus jannaschii |
| SAM-dependent N1-pseudouridine methyltransferase | - |
Methanocaldococcus jannaschii |
| TrmY protein | - |
Haloferax volcanii |
| TrmY protein | - |
Halobacterium sp. |
| TrmY protein | - |
Methanocaldococcus jannaschii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | m1PSI formation in archaeal tRNAs, overview. The enzyme belongs to Cluster of Orthologous Group, COG, 1901 | Haloferax volcanii |
| evolution | m1PSI formation in archaeal tRNAs, overview. The enzyme belongs to Cluster of Orthologous Group, COG, 1901 | Halobacterium sp. |
| evolution | m1PSI formation in archaeal tRNAs, overview. The enzyme belongs to Cluster of Orthologous Group, COG, 1901 | Methanocaldococcus jannaschii |
| malfunction | deletion of trmY leads to the absence of m1PSI54 in Haloferax volcanii tRNA | Haloferax volcanii |
| metabolism | Pus10-mediated PSI54 (and PSI55) formation does not require the presence of TrmY activity, overview | Haloferax volcanii |
| metabolism | Pus10-mediated PSI54 (and PSI55) formation does not require the presence of TrmY activity, overview | Methanocaldococcus jannaschii |
| additional information | Methanocaldococcus jannaschii TrmY requires C55 (or possibly U55) in the tRNA to produce m1PSI54 | Methanocaldococcus jannaschii |
| physiological function | trmY is not essential in Haloferax volcanii | Haloferax volcanii |
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