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Literature summary for 2.1.1.249 extracted from

  • Soares, J.; Zhang, L.; Pitsch, R.; Kleinholz, N.; Jones, R.; Wolff, J.; Amster, J.; Green-Church, K.; Krzyck, J.
    The residue mass of L-pyrrolysine in three distinct methylamine methyltransferases (2005), J. Biol. Chem., 280, 36962-36969.
    View publication on PubMed
Search for more literature for 2.1.1.249

Cloned(Commentary)

Cloned (Comment) Organism
gene mtbB Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylamine + a [Co(I) methylamine-specific corrinoid protein] Methanosarcina barkeri
-
 a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?
dimethylamine + a [Co(I) methylamine-specific corrinoid protein] Methanosarcina barkeri MS / DSM 800
-
 a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri Q9P9N0 gene mtbB
-
Methanosarcina barkeri MS / DSM 800 Q9P9N0 gene mtbB
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by three different steps of anion exchange chromatography, adsorption and hydrophobic interaction chromatography Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein]
-
 Methanosarcina barkeri a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?
dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein]
-
 Methanosarcina barkeri MS / DSM 800 a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?
dimethylamine + a [Co(I) methylamine-specific corrinoid protein]
-
 Methanosarcina barkeri a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?
dimethylamine + a [Co(I) methylamine-specific corrinoid protein]
-
 Methanosarcina barkeri MS / DSM 800 a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?

Subunits

Subunits Comment Organism
More mass spectrometric structure analysis, overview, primary structure of MtbB Methanosarcina barkeri

Synonyms

Synonyms Comment Organism
dimethylamine:CoM methyltransferase
-
 Methanosarcina barkeri
DMA methyltransferase
-
 Methanosarcina barkeri
MtbB
-
 Methanosarcina barkeri

General Information

General Information Comment Organism
additional information single in-frame amber UAG codons are found in the genes encoding MtmB, MtbB, or MttB, the methyltransferases initiating methane formation from monomethylamine, dimethylamine, or trimethylamine, respectively, in certain Archaea. The amber codon codes for pyrrolysine, the 22nd genetically encoded amino acid found in nature Methanosarcina barkeri