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Literature summary for 2.1.1.249 extracted from
- Activation and reaction kinetics of the dimethylamine/coenzyme M methyltransfer in Methanosarcina barkeri strain Fusaro (1998), Eur. J. Biochem., 258, 597-602.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ATP | acts as a powerful allosteric effector on the methyltransferase reaction. ATP serves as the substrate for MAP, which, after being phosphorylated by ATP, operates in the reductive activation of the corrinoid methyltransferase | Methanosarcina barkeri |  |
| methyltransferase activating protein | MAP, dependent on. ATP serves as the substrate for MAP, which, after being phosphorylated by ATP, operates in the reductive activation of the corrinoid methyltransferase | Methanosarcina barkeri | |
| additional information | as isolated, DMA-MT is inactive, but it can enzymically be reactivated by methyltransferase activating protein, ATP, and hydrogenase | Methanosarcina barkeri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Monomethylamine | MMA, acts as competitive inhibitor of the reaction. The methylation is specifically reversed by MMA | Methanosarcina barkeri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Co(I) dimethylamine-specific corrinoid protein, prosthetic group | Methanosarcina barkeri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | Methanosarcina barkeri | - |
a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
| dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | Methanosarcina barkeri DSM 804 | - |
a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina barkeri | - |
Fusaro | - |
| Methanosarcina barkeri DSM 804 | - |
Fusaro | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme to homogeneity | Methanosarcina barkeri |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] = a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | mechanism of the dimethylamine/coenzyme M methyltransfer, detailed overview | Methanosarcina barkeri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.74 | - |
pH 7.4, 37°C | Methanosarcina barkeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | - |
Methanosarcina barkeri | a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
| dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | the methylation is specifically reversed by methylamine | Methanosarcina barkeri | a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
| dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | - |
Methanosarcina barkeri DSM 804 | a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
| dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] | the methylation is specifically reversed by methylamine | Methanosarcina barkeri DSM 804 | a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dimethylamine/5-hydroxybenzimidazolylcobamide methyltransferase | - |
Methanosarcina barkeri |
| DMA-MT | - |
Methanosarcina barkeri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Methanosarcina barkeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Methanosarcina barkeri |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Co(I) dimethylamine-specific corrinoid protein | corrinoid prosthetic group | Methanosarcina barkeri |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the methyl group bound to the corrinoid prosthetic group of DMA-MT is subsequently transferred to coenzyme M in a reaction mediated by methylcobalamin/coenzyme M methyltransferase isoenzyme II, EC 2.1.1.247, which binds with high affinity to DMA-MT | Methanosarcina barkeri |
| additional information | as-isolated DMA-MT is inactive. The enzyme can be reactivated to yield the methylated B12-HBI prosthetic group by a system that comprises a methyl donor, a reductant, ATP and one (or more) component(s) present in the partially purified methyltransferase activating protein, MAP, fraction | Methanosarcina barkeri |
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