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Literature summary for 2.1.1.247 extracted from

  • Asakawa, S.; Sauer, K.; Liesack, W.; Thauer, R.
    Tetramethylammonium:coenzyme M methyltransferase system from Methanococcoides sp. (1998), Arch. Microbiol., 170, 220-226.
    View publication on PubMed
Search for more literature for 2.1.1.247

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri
-
 gene mtbA
-
Methanosarcina barkeri NaT1
-
 gene mtbA
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:trimethylamine-grown cell
-
 Methanosarcina barkeri
-
additional information cell extracts of strain NaT1 grown on trimethylamine rather than on tetramethylammonium are devoid of tetramethylammonium:coenzyme M methyltransferase activity Methanosarcina barkeri
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
 trimethylamine:coenzyme M methyltransferase activity, pH 7.0, 37°C Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme exhibits MT2 activity, the MT2 activity is measured by following the coenzyme-M-dependent demethylation of methylcobalamin Methanosarcina barkeri ?
-
 ?
additional information the enzyme exhibits MT2 activity, the MT2 activity is measured by following the coenzyme-M-dependent demethylation of methylcobalamin Methanosarcina barkeri NaT1 ?
-
 ?
[methyl-Co(III) trimethylamine-specific corrinoid protein] + coenzyme M
-
 Methanosarcina barkeri methyl-CoM + [Co(I) trimethylamine-specific corrinoid protein]
-
 ?
[methyl-Co(III) trimethylamine-specific corrinoid protein] + coenzyme M
-
 Methanosarcina barkeri NaT1 methyl-CoM + [Co(I) trimethylamine-specific corrinoid protein]
-
 ?

Synonyms

Synonyms Comment Organism
mtbA
-
 Methanosarcina barkeri
trimethylamine:coenzyme M methyltransferase
-
 Methanosarcina barkeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Methanosarcina barkeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Methanosarcina barkeri

General Information

General Information Comment Organism
additional information cell extracts of strain NaT1 catalyze the formation of methyl-coenzyme M from coenzyme M and tetramethylammonium, EC 2.1.1.253, or trimethylamine, EC 2.1.1.250, but not from coenzyme M and dimethylamine, EC 2.1.1.249, monomethylamine, EC 2.1.1.248, or methanol, EC 2.1.1.246 Methanosarcina barkeri