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Literature summary for 2.1.1.245 extracted from

  • Maupin-Furlow, J.; Ferry, J.
    Characterization of the cdhD and cdhE genes encoding subunits of the corrinoid/iron-sulfur enzyme of the CO dehydrogenase complex from Methanosarcina thermophila (1996), J. Bacteriol., 178, 340-346.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.245

Cloned(Commentary)

Cloned (Comment) Organism
genes cdhE and cdhD, the cdhD gene is located upstream of cdhE, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) Methanosarcina thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ CdhE contains approximately 4.5 mol of Fe, 2.7 mol of acid-labile sulfur, and 0.3 mol of hydroxocobalamin per mol of purified CdhE Methanosarcina thermophila
Fe2+ CdhE contains approximately 4.5 mol of Fe, 2.7 mol of acid-labile sulfur, and 0.3 mol of hydroxocobalamin per mol of purified CdhE Methanosarcina thermophila
Fe2+ in the iron-sulfur protein Methanosarcina thermophila

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila Q50538 CdhD1; genes cdhD and cdhE
-
Methanosarcina thermophila Q50539 CdhE1; genes cdhD and cdhE
-

Purification (Commentary)

Purification (Comment) Organism
recombinant CdhD from Escherichia coli strain BL21(DE3) Methanosarcina thermophila
recombinant CdhE from Escherichia coli strain BL21(DE3) Methanosarcina thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information reduction of the cobalt atom of the corrinoid to Co1+ by Ti(III)citrate, the gamma subunit CdhE contains the single 4Fe-4S center in the C/Fe-S enzyme Methanosarcina thermophila ?
-
 ?

Subunits

Subunits Comment Organism
More the C/Fe-S enzyme purified contains one molecule of factor III in the base-off form which facilitates reduction of the cobalt atom to the Co1+ redox state, which is a requirement for methylation Methanosarcina thermophila

Synonyms

Synonyms Comment Organism
C/Fe-S enzyme
-
 Methanosarcina thermophila
CdhD1
-
 Methanosarcina thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
13
-
 assay at Methanosarcina thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Methanosarcina thermophila

General Information

General Information Comment Organism
additional information genes cdhD and cdhE encode the delta and gamma subunits of the C/Fe-S enzyme from the CO dehydrogenase complex of Methanosarcina thermophila, the complex comprises a nickel/iron-sulfur, Ni/Fe-S, enzyme containing alpha and epsilon subunits and a corrinoid/iron-sulfur, C/Fe-S, enzyme containing gamma and delta subunits. The C/Fe-S enzyme contains one molecule of factor III in the base-off form which facilitates reduction of the cobalt atom to the Co1+ redox state, which is a requirement for methylation. The gamma subunit CdhE contains the single 4Fe-4S center in the C/Fe-S enzyme, overview Methanosarcina thermophila
additional information genes cdhD and cdhE encode the delta and gamma subunits of the C/Fe-S enzyme from the CO dehydrogenase complex of Methanosarcina thermophila, the complex comprises a nickel/iron-sulfur, Ni/Fe-S, enzyme containing alpha and epsilon subunits and a corrinoid/iron-sulfur, C/Fe-S, enzyme containing gamma and delta subunits. The C/Fe-S enzyme purified contains one molecule of factor III in the base-off form which facilitates reduction of the cobalt atom to the Co1+ redox state, which is a requirement for methylation. The gamma subunit CdhE contains the single 4Fe-4S center in the C/Fe-S enzyme Methanosarcina thermophila