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Literature summary for 2.1.1.243 extracted from

  • Braun, S.D.; Hofmann, J.; Wensing, A.; Ullrich, M.S.; Weingart, H.; Voelksch, B.; Spiteller, D.
    Identification of the biosynthetic gene cluster for 3-methylarginine, a toxin produced by Pseudomonas syringae pv. syringae 22d/93 (2010), Appl. Environ. Microbiol., 76, 2500-2508.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.243

Cloned(Commentary)

Cloned (Comment) Organism
gene mrsA, DNA and amino acid sequence determination and analysis, sequences of genes mrsA and mrsB reveal an overlapping region in the start/stop sequences, sequence comparisons, mrsA-ATGA-mrsB, overexpression of the His-tagged MrsA in Escherichia coli strain BL21(DE3) Pseudomonas syringae

Protein Variants

Protein Variants Comment Organism
additional information Tn5 transposon mutagenesis Pseudomonas syringae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 5-guanidino-2-oxopentanoate Pseudomonas syringae
-
 S-adenosyl-L-homocysteine + 5-guanidino-3-methyl-2-oxopentanoate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas syringae D5FKJ3 pv. syringae 22d/93
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged MrsA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Pseudomonas syringae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 5-guanidino-2-oxopentanoate
-
 Pseudomonas syringae S-adenosyl-L-homocysteine + 5-guanidino-3-methyl-2-oxopentanoate
-
 ?
S-adenosyl-L-methionine + 5-guanidino-2-oxopentanoate
-
 Pseudomonas syringae S-adenosyl-L-homocysteine + 5-guanidino-3-methyl-2-oxopentanoate NMR and mass spectrometric product analysis ?

Synonyms

Synonyms Comment Organism
mrsA
-
 Pseudomonas syringae
S-adenosylmethionine-dependent methyltransferase
-
 Pseudomonas syringae
SAM-dependent methyltransferase
-
 Pseudomonas syringae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay at Pseudomonas syringae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Pseudomonas syringae

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine dependent on Pseudomonas syringae

General Information

General Information Comment Organism
metabolism the enzyme is involved inthe biosynthesis of the rare amino acid 3-methylarginine Pseudomonas syringae
physiological function the enzyme is involved inthe biosynthesis of the rare amino acid 3-methylarginine, which is highly active against the closely related soybean pathogen Pseudomonas syringae pv. glycinea. Since these pathogens compete for the same habitat, Pss22d is a promising candidate for biocontrol of P. syringae pv. glycinea Pseudomonas syringae