Application | Comment | Organism |
---|---|---|
synthesis | introduction of enzyme into a resveratrol-producing Corynebacterium glutamicum strain allows synthesis of 42 mg/l (0.16 mM) of the di-O-methylated pterostilbene from p-coumaric acid. A fusion of O-methyltransferase with the maltose-binding protein of Escherichia coli lacking its signal peptide increases the solubility of the O-methyltransferase. Expression of heterologous dioxygenase genes in (2S)-flavanone-producing Corynebacterium glutamicum strains enables the production of flavanonols and flavonols starting from p-coumaric acid and caffeic acid. For the flavonols kaempferol and quercetin, maximum product titers of 23 mg/l (0.08 mM) and 10 mg/l (0.03 mM) can be achieved | Vitis vinifera |
synthesis | the enzyme is used for production of large amounts of easily recoverable extracellular resveratrol in a bacterial system, method, overview. Constitutive expression of either Vitis vinifera resveratrol O-methyltransferase (VvROMT) or human cytochrome P450 hydroxylase 1B1 (HsCYP1B1) lead to pterostilbene or piceatannol, respectively, after the engineered cell cultures are treated with elicitors. i.e. methylated cyclodextrins and methyl jasmonate. Functionality of both gene products is first assessed in planta by Nicotiana benthamiana agroinfiltration assays, in which tobacco cells transiently express stilbene synthase and VvROMT or HsCYP1B1 | Vitis vinifera |
Cloned (Comment) | Organism |
---|---|
gene ROMT, recombinant expression in Corynebacterium glutamicum and in Vitis vinifera cell culture, coexpression with flavanone 3-hydroxylase, and flavonol synthase, transient recombinant expression of HA-tagged enzyme in Nicotiana benthamiana leaves via Agrobacterium-mediated transient transformation, coexpression with human cytochrome P450 hydroxylase 1B1 (HsCYP1B1) | Vitis vinifera |
Protein Variants | Comment | Organism |
---|---|---|
additional information | functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum and in Vitis vinifera cell culture for production of pterostilbene, kaempferol, and quercetin | Vitis vinifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + trans-resveratrol | Vitis vinifera | - |
2 S-adenosyl-L-homocysteine + pterostilbene | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vitis vinifera | B6VJS4 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
berry | - |
Vitis vinifera | - |
cell culture | - |
Vitis vinifera | - |
fruit | - |
Vitis vinifera | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + trans-resveratrol | - |
Vitis vinifera | 2 S-adenosyl-L-homocysteine + pterostilbene | - |
? | |
2 S-adenosyl-L-methionine + trans-resveratrol | - |
Vitis vinifera | 2 H+ + pterostilbene + 2 S-adenosyl-L-homocysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MT | - |
Vitis vinifera |
ROMT | - |
Vitis vinifera |
VvRO | - |
Vitis vinifera |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Vitis vinifera |
Organism | Comment | Expression |
---|---|---|
Vitis vinifera | methylated cyclodextrins and methyl jasmonate induce the enzyme, accumulation of stilbenoids in grapevine cell cultures upon elicitation | up |