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Literature summary for 2.1.1.236 extracted from

  • Kharel, M.K.; Lian, H.; Rohr, J.
    Characterization of the TDP-D-ravidosamine biosynthetic pathway: one-pot enzymatic synthesis of TDP-D-ravidosamine from thymidine-5-phosphate and glucose-1-phosphate (2011), Org. Biomol. Chem., 9, 1799-1808.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis synthesis of TDP-D-ravidosamine, necessary for future in vitro glycosylation assays. TDP-D-ravidosamine is the anticipated sugar donor substrate of RavGT (the glycosyltransferase that links D-ravidosamine to the polyketide derived backbone defuco-gilvocarcin V). Defuco-gilvocarcin V exhibits superior anticancer/antibacterial activities Streptomyces ravidus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces ravidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31060
-
2 * 31060, SDS-PAGE Streptomyces ravidus

Organism

Organism UniProt Comment Textmining
Streptomyces ravidus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces ravidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose RavNMT catalyzes the transfer of methyl groups from S-adenosyl-L-methionine to the amino nitrogen of in a sequential manner Streptomyces ravidus 2 S-adenosyl-L-homocysteine + TDP-3-dimethylamino-3,6-dideoxy-alpha-D-galactopyranose TDP-3-dimethylamino-3,6-dideoxy-alpha-D-galactopyranose i.e. TDP-D-ravidosamine ?

Subunits

Subunits Comment Organism
dimer 2 * 31060, SDS-PAGE Streptomyces ravidus

Synonyms

Synonyms Comment Organism
RavNMT
-
Streptomyces ravidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Streptomyces ravidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Streptomyces ravidus