Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Trm112 p | the presence of Trm112p dramatically improves the methyltransferase activity of Trm9p in vitro | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
co-expression of His-tagged Trm9p with untagged Trm112p in Escherichia coli strain BL21(DE3)pLysS, Trm9p/Trm112p complex formation | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | alternative mechanisms in formation of the 5-carboxymethyluridine34 side chain at wobble position, overview | ? | - |
? | |
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
gene TRM9 | - |
Purification (Comment) | Organism |
---|---|
co-purification of His-tagged Trm9p with untagged Trm112p as a complex from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | alternative mechanisms in formation of the 5-carboxymethyluridine34 side chain at wobble position, overview | Saccharomyces cerevisiae | ? | - |
? | |
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Trm9p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | 5-methoxycarbonylmethyl-uridine or 5-methoxycarbonylmethyl-2-thiouridine are absent in tRNAs in trm9DELTA or trm112DELTA mutants, while intermediates 5-carbamoylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine are accumulating | Saccharomyces cerevisiae |
metabolism | Trm9p and Trm112p function together at the final step in formation of 5-methoxycarbonylmethyl-uridine in tRNA by using the intermediate 5-carbamoylmethyluridine as a substrate | Saccharomyces cerevisiae |
physiological function | at position 34, the majority of yeast cytosolic tRNA species that have a uridine are modified to 5-carbamoylmethyluridine, 5-carbamoylmethyl-2'-O-methyluridine, 5-methoxycarbonylmethyl-uridine or 5-methoxycarbonylmethyl-2-thiouridine. The formation of 5-methoxycarbonylmethyl-uridine and 5-carbamoylmethyluridine side chains involves a complex pathway, where the last step in formation of mcm5 is a methyl esterification of 5-carboxymethyl dependent on the Trm9 and Trm112 proteins. Both Trm9 and Trm112 are required for the last step in formation of 5-methoxycarbonylmethyl side chains at wobble uridines | Saccharomyces cerevisiae |