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Literature summary for 2.1.1.229 extracted from
- Mammalian ALKBH8 possesses tRNA methyltransferase activity required for the biogenesis of multiple wobble uridine modifications implicated in translational decoding (2010), Mol. Cell. Biol., 30, 1814-1827.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| TRM112 | interaction of ALKBH8 with a small accessory protein, TRM112, is required to form a functional tRNA methyltransferase | Mus musculus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q80Y20 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | ALKBH8 and TRM112 form a functional tRNA methyltransferase complex (Trm112 is an accessory protein required for several methyltransferases) | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ALKBH8 | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Mus musculus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | mcm5U, mcm5Um, and mcm5s2U are detected in total tRNA from wild-type livers but are completely absent from total tRNA from Alkbh8-/- mice. Substantial amounts of cm5U, the putative unmethylated precursor of mcm5U, is detected in total tRNA from Alkbh8-/- mice but not in total tRNA from wild-type mice. Despite the complete loss of all of these uridine modifications, Alkbh8-/- mice appear normal. However, the selenocysteine-specific tRNA is aberrantly modified in the Alkbh8-/- mice, and for the selenoprotein Gpx1, a reduced recoding of the UGA stop codon to selenocysteine is observed | Mus musculus |
| physiological function | ALKBH8-mediated methylation is a prerequisite for the thiolation and 2'-O-ribose methylation that form 5-methoxycarbonylmethyl-2-thiouridine and 5-methoxycarbonylmethyl-2'-O-methyluridine, respectively | Mus musculus |
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