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Literature summary for 2.1.1.228 extracted from

  • Sakaguchi, R.; Giessing, A.; Dai, Q.; Lahoud, G.; Liutkeviciute, Z.; Klimasauskas, S.; Piccirilli, J.; Kirpekar, F.; Hou, Y.M.
    Recognition of guanosine by dissimilar tRNA methyltransferases (2012), RNA, 18, 1687-1701.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.228

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information single turnover kinetics and thermodynamic analysis of effect of different guanosine analogues on m1G37-tRNA synthesis, kinetic analysis, overview Escherichia coli
additional information
-
 additional information single turnover kinetics and thermodynamic analysis of effect of different guanosine analogues on m1G37-tRNA synthesis, kinetic analysis, overview Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + guanine37 in tRNA Escherichia coli
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
S-adenosyl-L-methionine + guanine37 in tRNA Methanocaldococcus jannaschii
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Methanocaldococcus jannaschii
-
-
-

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA substrate recognition and reaction mechanisms, overview Escherichia coli
a
S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA substrate recognition and reaction mechanisms, overview Methanocaldococcus jannaschii
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information recognition of N1 of G37 in tRNA is essential for translational fidelity in all biological domains, Trm5 shows a less rigid requirement of guanosine functional groups. Replacment of functional groups of G37 by guanosine analogues, i.e. deoxyG, 6-thioG, inosine, and 2-aminopurine, in MjtRNACys, to design the optimal substrate for Trm5 Methanocaldococcus jannaschii ?
-
 ?
additional information recognition of N1 of G37 in tRNA is essential for translational fidelity in all biological domains, TrmD shows a more rigid requirement of guanosine functional groups. Replacment of functional groups of G37 by guanosine analogues, i.e. deoxyG, 6-thioG, inosine, and 2-aminopurine, in EctRNALeu, to design the optimal substrate for TrmD. All but deoxyG of these analogs probed the Watson-Crick basepairing interface of G37 Escherichia coli ?
-
 ?
S-adenosyl-L-methionine + guanine36 in tRNALeu G36-substituted tRNA substrate Escherichia coli tRNALeu, Trm5 shows a lack of discrimination between the two sequences of G36 and G37 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine36 in tRNALeu
-
 ?
S-adenosyl-L-methionine + guanine36 in tRNALeu G36-substituted tRNA substrate Escherichia coli tRNALeu, TrmD shows a 90fold reduced catalytic efficiency, discrimination between the two sequences of G36 and G37 Escherichia coli S-adenosyl-L-homocysteine + N1-methylguanine36 in tRNALeu
-
 ?
S-adenosyl-L-methionine + guanine37 in tRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
S-adenosyl-L-methionine + guanine37 in tRNA
-
 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
S-adenosyl-L-methionine + guanine37 in tRNA Trm5 recognizes N1 and O6 of G37, but the exocyclic 2-amino group of G37 is dispensable for Trm5. Trm5 does not require G36 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
S-adenosyl-L-methionine + guanine37 in tRNA TrmD recognizes N1 and O6 of G37 and the exocyclic 2-amino group of G37 is important for TrmD, also TrmD requires G36 for synthesis of m1G37 Escherichia coli S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
S-adenosyl-L-methionine + guanine37 in tRNACys Methanococcus jannaschii tRNACys Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNACys
-
 ?
S-adenosyl-L-methionine + guanine37 in tRNALeu Escherichia coli tRNALeu Escherichia coli S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeu
-
 ?

Synonyms

Synonyms Comment Organism
TRM5
-
 Methanocaldococcus jannaschii
TrmD
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Escherichia coli
S-adenosyl-L-methionine
-
 Methanocaldococcus jannaschii

General Information

General Information Comment Organism
additional information S-adenosyl-methionine-dependent m1G37-tRNA methyltransferases rapidly screen tRNA by direct recognition of G37 in order to monitor the global state of m1G37-tRNA Escherichia coli
additional information S-adenosyl-methionine-dependent m1G37-tRNA methyltransferases rapidly screen tRNA by direct recognition of G37 in order to monitor the global state of m1G37-tRNA Methanocaldococcus jannaschii