Cloned (Comment) | Organism |
---|---|
gene tlyA, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mycobacterium tuberculosis | the 2'-O-methyltransferase TlyA from Mycobacterium tuberculosis methylates both nucleotide C1409 of 16S rRNA, EC 2.1.1.227, and C1920 of 23S rRNA | ? | - |
? | |
additional information | Mycobacterium tuberculosis H37Rv | the 2'-O-methyltransferase TlyA from Mycobacterium tuberculosis methylates both nucleotide C1409 of 16S rRNA, EC 2.1.1.227, and C1920 of 23S rRNA | ? | - |
? | |
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA | Mycobacterium tuberculosis | - |
S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA | Mycobacterium tuberculosis H37Rv | - |
S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WJ63 | gene tlyA | - |
Mycobacterium tuberculosis H37Rv | P9WJ63 | gene tlyA | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged enzyme from Escherichia coli | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the 2'-O-methyltransferase TlyA from Mycobacterium tuberculosis methylates both nucleotide C1409 of 16S rRNA, EC 2.1.1.227, and C1920 of 23S rRNA | Mycobacterium tuberculosis | ? | - |
? | |
additional information | the purified recombinant enzyme TlyA efficiently 2'-O-methylates C1920 in vitro at a molar enzyme to substrate ratio of 1:10 | Mycobacterium tuberculosis | ? | - |
? | |
additional information | the 2'-O-methyltransferase TlyA from Mycobacterium tuberculosis methylates both nucleotide C1409 of 16S rRNA, EC 2.1.1.227, and C1920 of 23S rRNA | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
additional information | the purified recombinant enzyme TlyA efficiently 2'-O-methylates C1920 in vitro at a molar enzyme to substrate ratio of 1:10 | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA | - |
Mycobacterium tuberculosis | S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA | - |
Mycobacterium tuberculosis H37Rv | S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2'-O-methyltransferase | - |
Mycobacterium tuberculosis |
TlyA | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
additional information | the S4-type RNA recognition domain (residues 5-64), S-adenosyl-methionine binding domain (residues 84-153), SAM-interacting residues (residues 90-94), and four catalytic residues (K69-D154-K182-E238) are conserved in 2'-O-methyltrasferases TlyA | Mycobacterium tuberculosis |
physiological function | bacterial 2'-O-methyltransferase TlyA methylates either both nucleotide C1409 of 16S rRNA and C1920 of 23S rRNA. Both ribosomal methylations increase bacterial susceptibility to ribosome targeting antibiotics capreomycin and viomycin. The enzyme also function as a hemolysin, but increased bacterial hemolytic function is not likely a consequence of TlyA-mediated methylations of the ribosome | Mycobacterium tuberculosis |