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Literature summary for 2.1.1.224 extracted from

  • Hansen, L.H.; Planellas, M.H.; Long, K.S.; Vester, B.
    The order Bacillales hosts functional homologs of the worrisome cfr antibiotic resistance gene (2012), Antimicrob. Agents Chemother., 56, 3563-3567.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.224

Cloned(Commentary)

Cloned (Comment) Organism
gene clbA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain AS19 using plasmid pLJ10 Bacillus amyloliquefaciens
gene clbB, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain AS19 using plasmid pLJ10 Brevibacillus brevis
gene clbC, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain AS19 using plasmid pLJ10 Alkalihalobacillus clausii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA Bacillus amyloliquefaciens
-
 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
 ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA Brevibacillus brevis
-
 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
 ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA Alkalihalobacillus clausii
-
 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
 ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA Bacillus amyloliquefaciens FZB42
-
 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
 ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA Brevibacillus brevis NBRC 100599
-
 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Alkalihalobacillus clausii Q5WJ42 or strain domuvar, gene clbC, a cfr-like gene
-
Bacillus amyloliquefaciens
-
 gene clbA, a cfr-like gene
-
Bacillus amyloliquefaciens FZB42
-
 gene clbA, a cfr-like gene
-
Brevibacillus brevis C0ZJA6 gene clbB, a cfr-like gene
-
Brevibacillus brevis NBRC 100599 C0ZJA6 gene clbB, a cfr-like gene
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Bacillus amyloliquefaciens S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
 ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Brevibacillus brevis S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
 ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Alkalihalobacillus clausii S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
 ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Bacillus amyloliquefaciens S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA verification of RNA methylation at A2503 in 23S rRNA by primer extension method ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Brevibacillus brevis S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA verification of RNA methylation at A2503 in 23S rRNA by primer extension method ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Alkalihalobacillus clausii S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA verification of RNA methylation at A2503 in 23S rRNA by primer extension method ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Bacillus amyloliquefaciens FZB42 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
 ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Bacillus amyloliquefaciens FZB42 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA verification of RNA methylation at A2503 in 23S rRNA by primer extension method ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Brevibacillus brevis NBRC 100599 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
 ?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
 Brevibacillus brevis NBRC 100599 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA verification of RNA methylation at A2503 in 23S rRNA by primer extension method ?

Synonyms

Synonyms Comment Organism
ClbA
-
 Bacillus amyloliquefaciens
ClbB
-
 Brevibacillus brevis
ClbC
-
 Alkalihalobacillus clausii

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Bacillus amyloliquefaciens
S-adenosyl-L-methionine
-
 Brevibacillus brevis
S-adenosyl-L-methionine
-
 Alkalihalobacillus clausii

General Information

General Information Comment Organism
evolution ClbA acts via the same mechanism as the Cfr methyltransferase Bacillus amyloliquefaciens
evolution ClbB acts via the same mechanism as the Cfr methyltransferase Brevibacillus brevis
evolution ClbC acts via the same mechanism as the Cfr methyltransferase Alkalihalobacillus clausii
physiological function ClbA confers resistance to antibiotics, florfenicol, clindamycin, linezolid, tiamulin, and streptogramin A/streptogramin B, to the cell, also when expressed in Escherichia coli strain AS19, overview Bacillus amyloliquefaciens
physiological function ClbB confers resistance to antibiotics, florfenicol, clindamycin, linezolid, tiamulin, and streptogramin A/streptogramin B, to the cell, also when expressed in Escherichia coli strain AS19, overview Brevibacillus brevis
physiological function ClbC confers resistance to antibiotics, florfenicol, clindamycin, linezolid, tiamulin, and streptogramin A/streptogramin B, to the cell, also when expressed in Escherichia coli strain AS19, overview Alkalihalobacillus clausii