Cloned (Comment) | Organism |
---|---|
gene aq_311, recombinant expression in Escherichia coli strain Rosetta (DE3) | Aquifex aeolicus |
Crystallization (Comment) | Organism |
---|---|
purified enzyme in complex with S-adenosyl-L-methionine, sitting drop vapor diffusion method, mixing of 0.001 ml of 10-12 mg/ml protein in 20 mM Tris-HCl buffer, pH 8.0, containing 150 mM NaCl, 1 mM DTT, and 2 mM S-adenosyl-L-methionine, with 0.001 ml of reservoir solution containing 0.1 M Tris-HCl buffer, pH 8.4, and 20% ethanol, at 20°C, cryoprotection in 0.1 M Tris-HCl buffer, pH 8.4, 20% ethanol, and 35% ethylene glycol, X-ray diffraction structure determination and analysis at 2.2 A resolution, molecular replacement method, using the coordinates of TrmI from Thermotoga maritima, PDB ID 1O54, as the starting model | Aquifex aeolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28700 | - |
x * 29000, recombinant enzyme, SDS-PAGE, x * 28700, about, sequence calculation | Aquifex aeolicus |
29000 | - |
x * 29000, recombinant enzyme, SDS-PAGE, x * 28700, about, sequence calculation | Aquifex aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenine58 in tRNA | Aquifex aeolicus | - |
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | O66653 | gene aq_311 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain Rosetta (DE3) by heat treatment at 70°C for 30 min, anion exchange and hydrophobic interaction chromatography, and dialysis, followed by cation exchange chromatography, gel filtration, and ultrafiltration | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenine58 in tRNA | - |
Aquifex aeolicus | S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, recombinant enzyme, SDS-PAGE, x * 28700, about, sequence calculation | Aquifex aeolicus |
Synonyms | Comment | Organism |
---|---|---|
TrmI | - |
Aquifex aeolicus |
tRNA m1A58 methyltransferase | - |
Aquifex aeolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Aquifex aeolicus |
General Information | Comment | Organism |
---|---|---|
evolution | the N1-methyladenosine residue at position 58 of tRNA is found in the three domains of life | Aquifex aeolicus |
physiological function | the N1-methyladenosine residue at position 58 of tRNA is found in the three domains of life, and contributes to the stability of the three-dimensional L-shaped tRNA structure. In thermophilic bacteria, this modificationis important for thermal adaptation, and is catalyzed by thetRNA m1A58 methyltransferase TrmI, using S-adenosyl-L-methionine as the methyl donor | Aquifex aeolicus |