KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics for diverse tRNAPhe variants, overview | Thermus thermophilus | |
0.00013 | - |
adenine58 in tRNAPhe | pH 7.5-8.0, 55°C, wild-type Thermus thermophilus tRNAPhe as substrate | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenine58 in tRNA | Thermus thermophilus | - |
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA | - |
? | |
S-adenosyl-L-methionine + adenine58 in tRNA | Thermus thermophilus DSM 7039 | - |
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q8GBB2 | HB27 | - |
Thermus thermophilus DSM 7039 | Q8GBB2 | HB27 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenine58 in tRNA | - |
Thermus thermophilus | S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA | - |
? | |
S-adenosyl-L-methionine + adenine58 in tRNA | - |
Thermus thermophilus DSM 7039 | S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA | - |
? | |
S-adenosyl-L-methionine + adenine58 in tRNAGGUThr | the tRNA from Thermus thermophilus, that contains C60 instead of U60, is poorly methylated. Nucleoside analysis of tRNAGGUThr from the wild-type strain indicates that less than 50% of tRNAGGUThr contain m1A58 | Thermus thermophilus | S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA | - |
? | |
S-adenosyl-L-methionine + adenine58 in tRNAGGUThr | the tRNA from Thermus thermophilus, that contains C60 instead of U60, is poorly methylated. Nucleoside analysis of tRNAGGUThr from the wild-type strain indicates that less than 50% of tRNAGGUThr contain m1A58 | Thermus thermophilus DSM 7039 | S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA | - |
? | |
S-adenosyl-L-methionine + adenine58 in tRNAPhe | the Thermus thermophilus tRNAPhe transcript is methylated efficiently by the Thermus thermophilus enzyme, whereas the Saccharomyces cerevisiae tRNAPhe transcript is poorly methylated. Analysis of fourteen chimeric tRNA transcripts derived from these two tRNA reveals that enzyme TrmI recognized the combination of aminoacyl stem, variable region, and T-loop. TrmI methylates deltion transcripts still containing the aminoacyl stem, variable region, and T-arm. Positive sequence determinants are C56, purine 57, A58, and U60. Replacing A58 with inosine and 2-aminopurine completely abrogates methylation, demonstrating that the 6-amino group in A58 is recognized by enzyme TrmI | Thermus thermophilus | S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNAPhe | - |
? | |
S-adenosyl-L-methionine + adenine58 in tRNAPhe | the Thermus thermophilus tRNAPhe transcript is methylated efficiently by the Thermus thermophilus enzyme, whereas the Saccharomyces cerevisiae tRNAPhe transcript is poorly methylated. Analysis of fourteen chimeric tRNA transcripts derived from these two tRNA reveals that enzyme TrmI recognized the combination of aminoacyl stem, variable region, and T-loop. TrmI methylates deltion transcripts still containing the aminoacyl stem, variable region, and T-arm. Positive sequence determinants are C56, purine 57, A58, and U60. Replacing A58 with inosine and 2-aminopurine completely abrogates methylation, demonstrating that the 6-amino group in A58 is recognized by enzyme TrmI | Thermus thermophilus DSM 7039 | S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNAPhe | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TrmI | - |
Thermus thermophilus |
tRNA (m1A58) methyltransferase | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | assay at | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Thermus thermophilus |