Cloned (Comment) | Organism |
---|---|
genes GCD10 and GCD14 encoding TRM6 and TRM61, expression of His-tagged proteins in Escherichia coli strain Rosetta (DE3) | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged TRM6-TRM61 complex, from 0.1 M KCl, 0.1 M Tris-HCl, pH 8.0, 25% w/v PEG 2000 MME, X-ray diffraction structure determination and analysis at 2.8 A resolution, molecular replacement | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | TRM6-TRM61 complex | Saccharomyces cerevisiae | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenine58 in tRNA | Saccharomyces cerevisiae | TRM6 and TRM61 which compose a tRNA m1A58 methyltransferase that methylates m1A58 in tRNAs, especially tRNAiMe | S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
genes GCD10 and GCD14 encode TRM6 and TRM61, respectively | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged TRM6 and TRM61 from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography and gel filtration | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + adenine58 in tRNA = S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA | TRM61 is the subunit that binds S-adenosyl-l-methionine and both subunits contribute to target tRNA binding, catalytic mechanism, overview | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenine58 in tRNA | TRM6 and TRM61 which compose a tRNA m1A58 methyltransferase that methylates m1A58 in tRNAs, especially tRNAiMe | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA | - |
? | |
S-adenosyl-L-methionine + adenine58 in tRNA | adenine at position 58 is the most conserved nucleoside in tRNA and is located in the TpsiC loop | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tRNA m1A58 methyltransferase | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
physiological function | TRM6 and TRM61 compose a tRNA methyltransferase which catalyzes the methylation of the N1 of adenine at position 58 in tRNAs, especially initiator methionine tRNA. The TRM6-TRM61 complex is required specifically in the initiation step of translation because of a strong requirement for m1A58 in the processing and accumulation of tRNAiMet. It is also required for repression of translation of GCN4, a gene encoding a transcriptional activator of amino-acid biosynthetic enzymes, mRNA | Saccharomyces cerevisiae |