Cloned (Comment) | Organism |
---|---|
gene trmK, recombinant overexpression of N-terminally His6-tagged mutant enzyme C35S/C152S in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
C35S/C152S | site-directed mutagenesis, since wild-type TrmK is prone to aggregation through cysteine oxidation, a protein variant with cysteine to serine mutations (i.e. C35S and C152S) is produced, which shows abolished protein aggregation and retains full enzymatic activity | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenine22 in tRNA | Bacillus subtilis | - |
S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA | - |
? | |
S-adenosyl-L-methionine + adenine22 in tRNA | Bacillus subtilis 168 | - |
S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P54471 | - |
- |
Bacillus subtilis 168 | P54471 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged mutant enzyme C35S/C152S from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by thrombin, followed by ultrafiltration and gel filtration | Bacillus subtilis |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenine22 in tRNA | - |
Bacillus subtilis | S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA | - |
? | |
S-adenosyl-L-methionine + adenine22 in tRNA | - |
Bacillus subtilis 168 | S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | backbone 1H, 15N and 13C chemical shift assignments of TrmK from Bacillus subtilis obtained by heteronuclear multidimensional NMR spectroscopy as well as its secondary structure in solution, overview | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
TrmK | - |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
evolution | Bacillus subtilis TrmK belongs to the COG2384 (cluster of orthologous groups). The members of this family are found in Gram-negative and Gram-positive bacteria. Their sequences are well-conserved in many bacterial pathogens | Bacillus subtilis |
additional information | NMR chemical shift mapping is used to get insight on the protein-RNA recognition mode | Bacillus subtilis |