Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Medicago truncatula |
Crystallization (Comment) | Organism |
---|---|
crystallized from a polyethylene glycol precipitant containing 2.5 mM of the reaction product S-adenosyl-L-homocysteine, 2.5 A resolution | Medicago truncatula |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0733 | - |
2,7,4'-trihydroxyisoflavanone | pH 8.0, 30°C | Medicago truncatula | |
0.0998 | - |
S-adenosyl-L-methionine | pH 8.0, 30°C | Medicago truncatula |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 2,7,4'-trihydroxyisoflavanone | Medicago truncatula | - |
S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago truncatula | Q29U70 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 2,7,4'-trihydroxyisoflavanone | - |
Medicago truncatula | S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone | - |
? | |
S-adenosyl-L-methionine + 2,7,4'-trihydroxyisoflavanone | the bifunctional enzyme also catalyzes the 3-O-methylation of (+)-6a-hydroxymaackiain, with slightly lower catalytic efficiency | Medicago truncatula | S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HI4'OMT/HM3OMT | bifunctional enzyme | Medicago truncatula |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Medicago truncatula |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Medicago truncatula |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
334 | - |
2,7,4'-trihydroxyisoflavanone | pH 8.0, 30°C | Medicago truncatula |