Cloned (Comment) | Organism |
---|---|
phylogenetic analysis, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C79A | site-directed mutagenesis, inactive mutant | Homo sapiens |
E119A | site-directed mutagenesis, inactive mutant | Homo sapiens |
K122A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Homo sapiens |
K168A | site-directed mutagenesis, the mutant shows only slightly reduced activity compared to the wild-type enzyme | Homo sapiens |
K196A | site-directed mutagenesis, the mutant shows only slightly reduced activity compared to the wild-type enzyme | Homo sapiens |
K241A | site-directed mutagenesis, the mutant shows moderately reduced activity compared to the wild-type enzyme | Homo sapiens |
K251A | site-directed mutagenesis, the mutant shows moderately reduced activity compared to the wild-type enzyme | Homo sapiens |
K254A | site-directed mutagenesis, the mutant shows only slightly reduced activity compared to the wild-type enzyme | Homo sapiens |
K271A | site-directed mutagenesis, the mutant shows only slightly reduced activity compared to the wild-type enzyme | Homo sapiens |
K295A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Homo sapiens |
K346A | site-directed mutagenesis, the mutant shows only slightly reduced activity compared to the wild-type enzyme | Homo sapiens |
K363A | site-directed mutagenesis, the mutant shows only slightly reduced activity compared to the wild-type enzyme | Homo sapiens |
K367A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Homo sapiens |
K387A | site-directed mutagenesis, the mutant shows only slightly reduced activity compared to the wild-type enzyme | Homo sapiens |
R160A | site-directed mutagenesis, inactive mutant | Homo sapiens |
R162A | site-directed mutagenesis, inactive mutant | Homo sapiens |
R240A | site-directed mutagenesis, the mutant shows only slightly reduced activity compared to the wild-type enzyme | Homo sapiens |
R275A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Homo sapiens |
R288A | site-directed mutagenesis, the mutant shows moderately reduced activity compared to the wild-type enzyme | Homo sapiens |
R289A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Homo sapiens |
R369A | site-directed mutagenesis, the mutant shows moderately reduced activity compared to the wild-type enzyme | Homo sapiens |
R371A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Homo sapiens |
R84A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Homo sapiens |
R95A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytosine38 in tRNAAsp | Homo sapiens | - |
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli to over 90% purity, except for mutant R84A, which is about 75% pure | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | DNMT2 induces a conformational change in the tRNA of the DNMT2-tRNA complex | Homo sapiens | ? | - |
? | |
S-adenosyl-L-methionine + cytosine38 in tRNAAsp | - |
Homo sapiens | S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp | - |
? | |
S-adenosyl-L-methionine + cytosine38 in tRNAAsp | mapping of the tRNA binding site of DNMT2 by systematically mutating surface-exposed lysine and arginine residues to alanine and studying the tRNA methylation activity and binding of the corresponding variants. tRNA specificity determinants and tRNA binding pocket structure in the DNMT2, overview | Homo sapiens | S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structural modeling of DNMT2, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
DNMT2 methyltransferase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | DNMT2 methylates RNA by employing a DNA methyltransferase-like catalytic mechanism, which is clearly different from the mechanism of other RNA MTases. DNMT2 has changed its substrate specificity from DNA to RNA in the course of its evolution | Homo sapiens |