Literature summary for 2.1.1.204 extracted from

Goll, M.G.; Kirpekar, F.; Maggert, K.A.; Yoder, J.A.; Hsieh, C.L.; Zhang, X.; Golic, K.G.; Jacobsen, S.E.; Bestor, T.H.
Methylation of tRNAAsp by the DNA methyltransferase homolog Dnmt2 (2006), Science, 311, 395-398.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in NIH3T3 cells	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	human DNMT2 protein (hDNMT2) is primarily localized to the cytoplasm of transfected mouse 3T3 fibroblasts	Homo sapiens	5737	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + cytosine38 in tRNA	Homo sapiens	-	S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O14717	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + cytosine38 in tRNA	-	Homo sapiens	S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA	-	?
S-adenosyl-L-methionine + cytosine38 in tRNA	methylates tRNAAsp specifically at cytosine38 in the anticodon loop. Human DNMT2 protein restores methylation in vitro to tRNAAsp from Dnmt2-deficient strains of mouse, Arabidopsis thaliana, and Drosophila melanogaster in a manner that is dependent on preexisting patterns of modified nucleosides. Unmodified tRNAAsp produced by in vitro transcription is not a substrate for DNMT2, which suggests that methylation is guided to cytosine38 by other modifications. Mannosylqueuosine is likely to be involved, because it is unique to tRNAAsp. Analysis of tRNAAsp sequences show complete conservation of the anticodon loop in species whose genomes encode Dnmt2 homologs, but the tRNAAsp anticodon loops in Caenorhabditis elegans and Saccharomyces cerevisiae, which lack Dnmt2 homologs, have diverged	Homo sapiens	S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA	-	?

Synonyms

Synonyms	Comment	Organism
hDNMT2	-	Homo sapiens

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Release 2023.1 (January 2023)