Application | Comment | Organism |
---|---|---|
pharmacology | enzymes NSUN2 and METTL1 are implicated in 5-fluorouracil sensitivity in HeLa cells. Interfering with methylation of tRNAs might provide a promising rationale to improve 5-fluorouracil chemotherapy of cancer | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | combined knockdown of NSUN2 and METTL1, EC 2.1.1.33, in HeLa cells | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | NSUN2 is phosphorylated at Ser139 by Aurora-B to inhibit its enzymatic activity during mitosis | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
additional information | simultaneous overexpression of NSUN2 and METTL1, EC 2.1.1.33, is widely observed among human cancers | Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
NOP2/Sun domain family member 2 | - |
Homo sapiens |
NSUN2 | - |
Homo sapiens |
SAKI | - |
Homo sapiens |
substrate of AIM-1/Aurora kinase B | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | nonessential tRNA modifications by methyltransferases are evolutionarily conserved and have been reported to stabilize mature tRNA molecules and prevent rapid tRNA decay. The tRNA modifying enzymes, NSUN2 and METTL1, EC 2.1.1.33, are mammalian orthologues of yeast Trm4 and Trm8, which are required for protecting tRNA against tRNA decay | Homo sapiens |
malfunction | combined knockdown of NSUN2 and METTL1, EC 2.1.1.33, in HeLa cells drastically potentiate sensitivity of cells to 5-fluorouracil, but does not affect cisplatin- and paclitaxel-induced cytotoxicity, synergistic effects of NSUN2 and METTL1 double knockdown, which causes rapid tRNA(ValAAC) degradation induced by destabilizing 5-fluorouracil | Homo sapiens |
physiological function | NSUN2 (NOP2/Sun domain family, member 2) is a NOL1/NOP2/SUN domain-containing tRNA (cytosine-5-)-methyltransferase. Nonessential tRNA modifications by methyltransferases are evolutionarily conserved and have been reported to stabilize mature tRNA molecules and prevent rapid tRNA decay. The tRNA modifying enzyme NSUN2 is a mammalian orthologues of yeast Trm4, which is required for protecting tRNA against tRNA decay. Enzyme NUSN2 is not a critical regulator of cancer cell growth | Homo sapiens |