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Literature summary for 2.1.1.203 extracted from

  • Okamoto, M.; Fujiwara, M.; Hori, M.; Okada, K.; Yazama, F.; Konishi, H.; Xiao, Y.; Qi, G.; Shimamoto, F.; Ota, T.; Temme, A.; Tatsuka, M.
    tRNA modifying enzymes, NSUN2 and METTL1, determine sensitivity to 5-fluorouracil in HeLa cells (2014), PLoS Genet., 10, e1004639.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
pharmacology enzymes NSUN2 and METTL1 are implicated in 5-fluorouracil sensitivity in HeLa cells. Interfering with methylation of tRNAs might provide a promising rationale to improve 5-fluorouracil chemotherapy of cancer Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information combined knockdown of NSUN2 and METTL1, EC 2.1.1.33, in HeLa cells Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein NSUN2 is phosphorylated at Ser139 by Aurora-B to inhibit its enzymatic activity during mitosis Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
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Homo sapiens
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additional information simultaneous overexpression of NSUN2 and METTL1, EC 2.1.1.33, is widely observed among human cancers Homo sapiens
-

Synonyms

Synonyms Comment Organism
NOP2/Sun domain family member 2
-
Homo sapiens
NSUN2
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Homo sapiens
SAKI
-
Homo sapiens
substrate of AIM-1/Aurora kinase B
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Homo sapiens

General Information

General Information Comment Organism
evolution nonessential tRNA modifications by methyltransferases are evolutionarily conserved and have been reported to stabilize mature tRNA molecules and prevent rapid tRNA decay. The tRNA modifying enzymes, NSUN2 and METTL1, EC 2.1.1.33, are mammalian orthologues of yeast Trm4 and Trm8, which are required for protecting tRNA against tRNA decay Homo sapiens
malfunction combined knockdown of NSUN2 and METTL1, EC 2.1.1.33, in HeLa cells drastically potentiate sensitivity of cells to 5-fluorouracil, but does not affect cisplatin- and paclitaxel-induced cytotoxicity, synergistic effects of NSUN2 and METTL1 double knockdown, which causes rapid tRNA(ValAAC) degradation induced by destabilizing 5-fluorouracil Homo sapiens
physiological function NSUN2 (NOP2/Sun domain family, member 2) is a NOL1/NOP2/SUN domain-containing tRNA (cytosine-5-)-methyltransferase. Nonessential tRNA modifications by methyltransferases are evolutionarily conserved and have been reported to stabilize mature tRNA molecules and prevent rapid tRNA decay. The tRNA modifying enzyme NSUN2 is a mammalian orthologues of yeast Trm4, which is required for protecting tRNA against tRNA decay. Enzyme NUSN2 is not a critical regulator of cancer cell growth Homo sapiens