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Literature summary for 2.1.1.203 extracted from

  • Yuan, S.; Tang, H.; Xing, J.; Fan, X.; Cai, X.; Li, Q.; Han, P.; Luo, Y.; Zhang, Z.; Jiang, B.; Dou, Y.; Gorospe, M.; Wang, W.
    Methylation by NSun2 represses the levels and function of microRNA 125b (2014), Mol. Cell. Biol., 34, 3630-3641.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.203

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged NSun2 in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information siRNA-mediated knockdown of NSun2 in HeLa cells Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + microRNA 125b Homo sapiens the tRNA methyltransferase NSun2 methylates primary (pri-miR-125b), precursor (pre-miR-125b), and mature microRNA 125b (miR-125b) in vitro and in vivo S-adenosyl-L-homocysteine + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged NSun2 from Escherichia coli by nickel affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + microRNA 125b the tRNA methyltransferase NSun2 methylates primary (pri-miR-125b), precursor (pre-miR-125b), and mature microRNA 125b (miR-125b) in vitro and in vivo Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?

Synonyms

Synonyms Comment Organism
NSUN2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function methylation by NSun2 inhibits the processing of pri-miR- 125b2 into pre-miR-125b2, decreases the cleavage of pre-miR-125b2 into miR-125, and attenuates the recruitment of RISC by miR-125, thereby repressing the function of miR-125b in silencing gene expression Homo sapiens