Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chromatoid body | - |
Mus musculus | 33391 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | NSun2 catalyzes the formation of cytosine-5 methylation in several tRNAs in vivo in tissues, including skin, liver, and testis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
germ cell | - |
Mus musculus | - |
liver | - |
Mus musculus | - |
skin | - |
Mus musculus | - |
testis | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | NSun2 catalyzes the formation of cytosine-5 methylation in several tRNAs in vivo in tissues, including skin, liver, and testis | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytosine-5 RNA methyltransferase | - |
Mus musculus |
NSUN2 | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | in the absence of NSun2, tRNAs lack specific cytosine-5 methylation modifications, which can cause reduced protein translation rates. In NSun2-depleted testes, genes encoding Ddx4, Miwi, and Tudor domain-containing (Tdr) proteins are repressed, indicating that RNA-processing and posttranscriptional pathways are impaired. Proteins of the RNA-processing machinery are reduced in NSun2-/- testes. Loss of NSun2 specifically blocks meiotic progression of germ cells into the pachytene stage, as spermatogonial and Sertoli cells are unaffected in knockout mice. Lack of NSun2 causes a block of progression of the first prophase of male meiosis at the zygotene-pachytene stage before the chromatoid bodies first appear in the cytoplasm at the late pachytene stage. Phenotype, detailed overview | Mus musculus |
physiological function | NSun2 catalyzes the formation of cytosine-5 methylation in several tRNAs in vivo in tissues, including skin, liver, and testis. Functionally, the cytosine-5 methylation posttranscriptional modification influences translation rates, as well as correct RNA folding and stability. The mouse enzyme NSun2 is a component of the chromatoid body, NSun2 is essential for germ cell differentiation in the mouse testis. Cytosine-5 tRNA methyltransferases are dispensable for the spermatogonium and early spermatocytes | Mus musculus |