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Literature summary for 2.1.1.202 extracted from

  • Hussain, S.; Sajini, A.A.; Blanco, S.; Dietmann, S.; Lombard, P.; Sugimoto, Y.; Paramor, M.; Gleeson, J.G.; Odom, D.T.; Ule, J.; Frye, M.
    NSun2-mediated cytosine-5 methylation of vault noncoding RNA determines its processing into regulatory small RNAs (2013), Cell Rep., 4, 255-261.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.202

Protein Variants

Protein Variants Comment Organism
C271A site-directed mutagenesis, the mutant enzyme forms an irreversible covalent crosslink between itself and the methylated cytosine in its target RNA Homo sapiens
additional information the only mRNA identified by miCLIP that is differentially expressed when NSun2 is inhibited by RNAi in HEK293 cells is NSun2 itself Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the iCLIP method identifies tRNA AspGTC, ValAAC, GlyGCC, and LeuCAA as methylation substrates with methylation within the variable arm at cytosines 48, 49, and 50, no additional NSun2 target sites outside the variable arm. vtRNAs are methylation substrates for NSun2, vtRNAs are ncRNAs found as part of the vault ribonucleoprotein complex ?
-
 ?
S-adenosyl-L-methionine + cytosine48 in tRNAAspGTC Homo sapiens
-
 S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAAspGTC
-
 ?
S-adenosyl-L-methionine + cytosine48 in tRNAGlyGCC Homo sapiens
-
 S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGlyGCC
-
 ?
S-adenosyl-L-methionine + cytosine48 in tRNALeuCAA Homo sapiens
-
 S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNALeuCAA
-
 ?
S-adenosyl-L-methionine + cytosine49 in tRNAAspGTC Homo sapiens
-
 S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAAspGTC
-
 ?
S-adenosyl-L-methionine + cytosine49 in tRNAGlyGCC Homo sapiens
-
 S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGlyGCC
-
 ?
S-adenosyl-L-methionine + cytosine49 in tRNALeuCAA Homo sapiens
-
 S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNALeuCAA
-
 ?
S-adenosyl-L-methionine + cytosine50 in tRNAAspGTC Homo sapiens
-
 S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAAspGTC
-
 ?
S-adenosyl-L-methionine + cytosine50 in tRNAGlyGCC Homo sapiens
-
 S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAGlyGCC
-
 ?
S-adenosyl-L-methionine + cytosine50 in tRNALeuCAA Homo sapiens
-
 S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNALeuCAA
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
skin fibroblast
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the iCLIP method identifies tRNA AspGTC, ValAAC, GlyGCC, and LeuCAA as methylation substrates with methylation within the variable arm at cytosines 48, 49, and 50, no additional NSun2 target sites outside the variable arm. vtRNAs are methylation substrates for NSun2, vtRNAs are ncRNAs found as part of the vault ribonucleoprotein complex Homo sapiens ?
-
 ?
additional information development and evaluation of a customized version of the individual-nucleotide-resolution crosslinking and immunoprecipitation (iCLIP) method for detection of cytosine methylation in RNA species, site-specific methylation in tRNAs and additional messenger and noncoding RNAs (ncRNAs), overview. Identified NSun2 targets are tRNAs, mRNAs, and ncRNAs Homo sapiens ?
-
 ?
S-adenosyl-L-methionine + cytosine48 in tRNAAspGTC
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAAspGTC
-
 ?
S-adenosyl-L-methionine + cytosine48 in tRNAGlyGCC
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGlyGCC
-
 ?
S-adenosyl-L-methionine + cytosine48 in tRNALeuCAA
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNALeuCAA
-
 ?
S-adenosyl-L-methionine + cytosine49 in tRNAAspGTC
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAAspGTC
-
 ?
S-adenosyl-L-methionine + cytosine49 in tRNAGlyGCC
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGlyGCC
-
 ?
S-adenosyl-L-methionine + cytosine49 in tRNALeuCAA
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNALeuCAA
-
 ?
S-adenosyl-L-methionine + cytosine50 in tRNAAspGTC
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAAspGTC
-
 ?
S-adenosyl-L-methionine + cytosine50 in tRNAGlyGCC
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAGlyGCC
-
 ?
S-adenosyl-L-methionine + cytosine50 in tRNALeuCAA
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNALeuCAA
-
 ?

Synonyms

Synonyms Comment Organism
NSUN2
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens sequenced cDNA libraries prepared from small RNA isolated from NSUN2+/- and NSUN2-/- human fibroblasts reveal that eight of the 43 ncRNAs are differentially regulated additional information

General Information

General Information Comment Organism
malfunction autosomal-recessive loss of the NSUN2 gene is a causative link to intellectual disability disorders in humans. Loss of cytosine-5 methylation in vault RNAs causes aberrant processing into Argonaute-associated small RNA fragments that can function as microRNAs. Impaired processing of vault ncRNA may contribute to the etiology of NSun2-deficiency human disorders Homo sapiens
physiological function no evidence for a major role of NSun2 or NSun2-mediated cytosine-5 methylation in mRNA stability Homo sapiens